Human CD161 Protein 3898

$345.00 – $1,150.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln67-Ser225

SKU: 3898parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q12918
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD161 (NKRP1) is a lectin-like receptor present on NK cells and rare T-cell subsets. We have observed CD161 expression in some cases of T-cell prolymphocytic leukemia (T-PLL) and found it to be useful in follow-up and detection of disease after treatment.
molecular weight	The protein has a predicted MW of 19.6 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human CD161 Protein 3898

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD161 (NKRP1) is a lectin-like receptor present on NK cells and rare T-cell subsets. We have observed CD161 expression in some cases of T-cell prolymphocytic leukemia (T-PLL) and found it to be useful in follow-up and detection of disease after treatment.
Protein names Killer cell lectin-like receptor subfamily B member 1 (C-type lectin domain family 5 member B) (HNKR-P1a) (NKR-P1A) (Natural killer cell surface protein P1A) (CD antigen CD161)
Protein family 349
Function Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.
Pathway 243
Subellular location Membrane ; Single-pass type II membrane protein .
Tissues Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype.
Structure Homodimer; disulfide-linked. Interacts with acid sphingomyelinase/SMPD1.
Post-translational modification N-glycosylated. Contains sialic acid residues.
Target Relevance information above includes information from UniProt accession: Q12918
The UniProt Consortium

Data

Anti-CD161 Antibody, hFc tag captured on CM5 Chip via Anti-hFc IgG can bind Human CD161, His Tag with an affinity constant of 78.8 nM as determined in SPR assay (Biacore T200).

Immobilized Human CD161, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Anti-CD161 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test).

The purity of Human CD161 is greater than 95% as determined by SEC-HPLC.

Human CD161 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.