Human IGFBP-3 Protein 3893

$203.00 – $675.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gly28-Lys291

SKU: 3893parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	AAH18962
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE
background	Insulin-like growth factor binding protein-3 (IGFBP-3) is a p53 tumor suppressor-regulated protein and a major carrier for IGFs in circulation. Among six high-affinity IGFBPs, which are IGFBP-1 through 6, IGFBP-3 is the most extensively investigated IGFBP species with respect to its IGF/IGF-I receptor (IGF-IR)-independent biological actions beyond its endocrine/paracrine/autocrine role in modulating IGF action in cancer.
molecular weight	The protein has a predicted MW of 29.8 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human IGFBP-3 Protein 3893

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Insulin-like growth factor binding protein-3 (IGFBP-3) is a p53 tumor suppressor-regulated protein and a major carrier for IGFs in circulation. Among six high-affinity IGFBPs, which are IGFBP-1 through 6, IGFBP-3 is the most extensively investigated IGFBP species with respect to its IGF/IGF-I receptor (IGF-IR)-independent biological actions beyond its endocrine/paracrine/autocrine role in modulating IGF action in cancer.
Protein names Insulin-like growth factor-binding protein 3 (IBP-3) (IGF-binding protein 3) (IGFBP-3)
Mass 31674Da
Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Inhibits the positive effect of humanin on insulin sensitivity (PubMed:19623253). Promotes testicular germ cell apoptosis (PubMed:19952275). {ECO:0000269\|PubMed:19623253, ECO:0000269\|PubMed:19952275, ECO:0000269\|PubMed:20353938}.
Subellular location Secreted {ECO:0000269\|PubMed:20353938}.
Tissues Expressed by most tissues. Present in plasma.
Structure Interacts with XLKD1 (By similarity). Binds IGF2 more than IGF1. Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS). Interacts with humanin; humanin competes with importin KPNB1 for binding to IGFBP3, blocking IGFBP3 nuclear import and IGFBP3-mediated apoptosis (PubMed:14561895, PubMed:19623253, PubMed:26216267). Interacts with TMEM219. {ECO:0000250, ECO:0000269\|PubMed:14561895, ECO:0000269\|PubMed:1726837, ECO:0000269\|PubMed:19623253, ECO:0000269\|PubMed:20353938, ECO:0000269\|PubMed:26216267}.
Post-translational modification Phosphorylated by FAM20C in the extracellular medium. {ECO:0000269\|PubMed:26091039}.
Domain The thyroglobulin type-1 domain mediates interaction with HN.
Target Relevance information above includes information from UniProt accession: P17936
The UniProt Consortium

Data

Human IGFBP-3, His Tag captured on CM5 Chip via anti-his antibody can bind Human IGFI, hFc Tag with an affinity constant of 2.32 nM as determined in SPR assay (Biacore T200).

Immobilized Human IGFBP-3, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human IGF-II, hFc Tag with the EC50 of 0.19µg/ml determined by ELISA (QC Test).

Human IGFBP-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.