Human Kallikrein 5/KLK5 Protein 3739

$300.00 – $1,000.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Val23-Ser293

SKU: 3739parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q9Y337
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-of-function mutations in the SPINK5 gene lead to down-regulation of the endogenous inhibitor LEKTI-1 and profound skin-barrier defects with severe allergic manifestations. To aid in the development of a medicine for this target, an X-ray crystallographic system was developed to facilitate fragment-guided chemistry and knowledge-based drug-discovery approaches.
molecular weight	The protein has a predicted MW of 30.7 kDa. Due to glycosylation, the protein migrates to 42-48 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human Kallikrein 5/KLK5 Protein 3739

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-of-function mutations in the SPINK5 gene lead to down-regulation of the endogenous inhibitor LEKTI-1 and profound skin-barrier defects with severe allergic manifestations. To aid in the development of a medicine for this target, an X-ray crystallographic system was developed to facilitate fragment-guided chemistry and knowledge-based drug-discovery approaches.
Protein names Kallikrein-5 (EC 3.4.21.-) (Kallikrein-like protein 2) (KLK-L2) (Stratum corneum tryptic enzyme)
Gene names KLK5,KLK5 SCTE UNQ570/PRO1132
Protein family Peptidase S1 family, Kallikrein subfamily
Mass 32020Da
Function May be involved in desquamation.
Subellular location Secreted.
Tissues Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin.
Structure Interacts with SPINK9.
Target Relevance information above includes information from UniProt accession: Q9Y337
The UniProt Consortium

Data

ELISA with Human Kallikrein 5/KLK5 Protein

Immobilized Human Kallikrein 5, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA.

The purity of Human Kallikrein 5 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human Kallikrein 5/KLK5 Protein

Human Kallikrein 5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.