ELISA with Human MDL-1/CLEC5A Protein 3705

Human MDL-1/CLEC5A Protein 3705

$300.00 – $1,000.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Tyr26-Lys188

SKU: 3705parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q9NY25
express system	HEK293
product tag	N-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	In C. elegans, increased lifespan in daf-2 insulin/IGF-1 receptor mutants is accompanied by up-regulation of the MDL-1 Mad basic helix-loop-helix leucine zipper transcription factor.MDL-1, like its mammalian orthologs, is an inhibitor of cell proliferation and growth that slows progression of an age-related pathology in C. elegans (uterine tumors). In addition, intestine-limited rescue of mdl-1 increased lifespan but not to wild type levels. Thus, mdl-1 likely acts both in the intestine and the germline to influence age-related mortality.
molecular weight	The protein has a predicted MW of 46 kDa. Due to glycosylation, the protein migrates to 63-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human MDL-1/CLEC5A Protein 3705

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
In C. elegans, increased lifespan in daf-2 insulin/IGF-1 receptor mutants is accompanied by up-regulation of the MDL-1 Mad basic helix-loop-helix leucine zipper transcription factor.MDL-1, like its mammalian orthologs, is an inhibitor of cell proliferation and growth that slows progression of an age-related pathology in C. elegans (uterine tumors). In addition, intestine-limited rescue of mdl-1 increased lifespan but not to wild type levels. Thus, mdl-1 likely acts both in the intestine and the germline to influence age-related mortality.
Protein names C-type lectin domain family 5 member A (C-type lectin superfamily member 5) (Myeloid DAP12-associating lectin 1) (MDL-1)
Gene names CLEC5A,CLEC5A CLECSF5 MDL1
Mass 9606Da
Function Functions as a positive regulator of osteoclastogenesis (By similarity). Cell surface receptor that signals via TYROBP (PubMed:10449773). Regulates inflammatory responses (By similarity).; (Microbial infection) Critical macrophage receptor for dengue virus serotypes 1-4 (PubMed:18496526, PubMed:21566123). The binding of dengue virus to CLEC5A triggers signaling through the phosphorylation of TYROBP (PubMed:18496526). This interaction does not result in viral entry, but stimulates pro-inflammatory cytokine release (PubMed:18496526).
Subellular location Cell membrane ; Single-pass type II membrane protein .
Tissues Highly expressed in bone marrow with lower levels in synovium, lung and bronchus (PubMed:20212065). Expressed in peripheral blood monocytes and in the monocyte/macrophage cell lines U-937 and Mono-Mac-6, but not in cell lines of other origins (PubMed:10449773). Expression is down-regulated when monocytes differentiate into dendritic cells (PubMed:10449773).
Structure Monomer (PubMed:21566123). Homodimer (PubMed:21566123). The majority of CLEC5A is expressed as a monomeric form on macrophages (PubMed:21566123). Interacts with TYROBP/DAP12 (PubMed:10449773). The interaction with TYROBP is required for CLEC5A cell surface expression (PubMed:10449773). Interacts with HCST/DAP10 (By similarity). Forms a CLEC5A/TYROBP/HCST trimolecular complex depending almost solely on TYROBP (By similarity).; (Microbial infection) Interacts with dengue virus envelope protein E (PubMed:18496526, PubMed:21566123).
Post-translational modification N-glycosylated. Contains sialic acid residues.
Target Relevance information above includes information from UniProt accession: Q9NY25
The UniProt Consortium

Immobilized Human MDL-1, hFc Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Anti-MDL-1 Antibody, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA.

The purity of Human MDL-1 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human MDL-1/CLEC5A Protein

Human MDL-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.