Mouse Siglec-4a/MAG Protein 3696

$240.00 – $800.00

Summary

Expression: HEK293
Pure: Yes (HPLC)
Amino Acid Range: Gly20-Pro516

SKU: 3696parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P20917
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2.
molecular weight	The protein has a predicted MW of 56 kDa. Due to glycosylation, the protein migrates to 70-85 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse Siglec-4a/MAG Protein 3696

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2.
Protein names Myelin-associated glycoprotein (Siglec-4a)
Gene names Mag,Mag
Protein family Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
Mass 10090Da
Function Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (PubMed:12089450, PubMed:27922006, PubMed:7533044). Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination (PubMed:10625334, PubMed:7516497, PubMed:9262180, PubMed:9469574, PubMed:9482781, PubMed:9482783). Protects motoneurons against apoptosis, also after injury; protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2 (PubMed:26335717). Required to prevent degeneration of myelinated axons in adults; this probably depends on binding to gangliosides on the axon cell membrane (PubMed:15953602, PubMed:19158290). Negative regulator of neurite outgrowth that inhibits axon longitudinal growth (PubMed:12089450, PubMed:19158290, PubMed:27922006). Negative regulator of neurite outgrowth; in dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides (PubMed:17640868). In cerebellar granule cells the inhibition is mediated via binding to neuronal gangliosides (PubMed:17640868). In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides (By similarity). Inhibits axon outgrowth by binding to RTN4R (PubMed:12089450). Preferentially binds to alpha-2,3-linked sialic acid (PubMed:27922006, PubMed:7533044). Binds ganglioside Gt1b (PubMed:27922006).
Subellular location Cell membrane ; Single-pass type I membrane protein. Membrane raft .
Tissues Detected in the myelin tract in brain, especially in the corpus callosum and in peripheral nerve (PubMed:24191038, PubMed:7516497, PubMed:9482783). Expressed by myelinating glial cells in the central and peripheral nervous system (PubMed:10625334). Detected in oligodendrocyte processes before formation of compact myelin (PubMed:10625334, PubMed:2474006). Restricted to the periaxonal space after myelination (PubMed:10625334). Isoform S-MAG is the predominant isoform in CNS and PNS of the adult (at protein level) (PubMed:1716323).
Structure Monomer and homodimer (PubMed:27922006). Interacts (via the first three N-terminal Ig-like domains) with RTN4R and RTN4RL2 (PubMed:12089450, PubMed:26335717). Interacts with isoform 2 of BSG (PubMed:12558975).
Post-translational modification N-glycosylated.; Phosphorylated on tyrosine residues.; Ubiquitinated, leading to proteasomal degradation.
Domain Th
Target Relevance information above includes information from UniProt accession: P20917
The UniProt Consortium

The purity of Mouse Siglec-4a is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Mouse Siglec-4a/MAG Protein

Mouse Siglec-4a on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.