Mouse IL-15RA/IL-15 R alpha/CD215 Protein 5129

$225.00 – $750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gly33-Lys205

SKU: 5129parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q60819
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.
molecular weight	The protein has a predicted MW of 45.1 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse IL-15RA/IL-15 R alpha/CD215 Protein 5129

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.
Protein names Interleukin-15 receptor subunit alpha (IL-15 receptor subunit alpha) (IL-15R-alpha) (IL-15RA) (CD antigen CD215) [Cleaved into: Soluble interleukin-15 receptor subunit alpha (sIL-15 receptor subunit alpha) (sIL-15R-alpha) (sIL-15RA)]
Gene names Il15ra,Il15ra
Mass 10090Da
Function High-affinity receptor for interleukin-15 (PubMed:17947230). Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells (PubMed:17947230). In neutrophils, binds and activates kinase SYK in response to IL15 stimulation (By similarity). In neutrophils, required for IL15-induced phagocytosis in a SYK-dependent manner (By similarity).
Subellular location Membrane ; Single-pass type I membrane protein. Nucleus membrane ; Single-pass type I membrane protein. Cell surface .; [Soluble interleukin-15 receptor subunit alpha]: Secreted, extracellular space .
Tissues Widely expressed.
Structure The interleukin-15 receptor IL15R is a heterotrimer of IL15RA, IL2RB and IL2RG. IL15RA also self-associates (By similarity). Interacts with SYK (By similarity).
Post-translational modification N-glycosylated and O-glycosylated.; A soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor (By similarity). It also binds IL15 and thus interferes with IL15 binding to the membrane receptor (By similarity).
Target Relevance information above includes information from UniProt accession: Q60819
The UniProt Consortium

ELISA with Mouse IL-15RA/IL-15 R alpha/CD215 Protein

Immobilized Human IL-15, No Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Mouse IL-15RA, hFc Tag with the EC50 of 55.6ng/ml determined by ELISA.

HPLC of Mouse IL-15RA/IL-15 R alpha/CD215 Protein

The purity of Mouse IL-15RA is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Mouse IL-15RA/IL-15 R alpha/CD215 Protein

Mouse IL-15RA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.