Biotinylated Human Siglec-4a/MAG Protein 3582

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gly20-Pro516

SKU: 3582parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity).
molecular weight	The protein has a predicted MW of 57.6 kDa. Due to glycosylation, the protein migrates to 78-85 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human Siglec-4a/MAG Protein 3582

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity).
Protein names Myelin-associated glycoprotein (Siglec-4a)
Gene names MAG,MAG GMA
Protein family Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
Mass 9606Da
Function Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity). Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. Protects motoneurons against apoptosis, also after injury; protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. Required to prevent degeneration of myelinated axons in adults; this probably depends on binding to gangliosides on the axon cell membrane (By similarity). Negative regulator of neurite outgrowth; in dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides. In cerebellar granule cells the inhibition is mediated primarily via binding to neuronal gangliosides. In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides. Inhibits axon longitudinal growth (By similarity). Inhibits axon outgrowth by binding to RTN4R (By similarity). Preferentially binds to alpha-2,3-linked sialic acid. Binds ganglioside Gt1b (By similarity).
Catalytic activity BINDING 65..67; /ligand="a ganglioside GT1b (d18:1(4E))"; /ligand_id="ChEBI:CHEBI:78452"; /evidence="ECO:0000250\|UniProtKB:P20917"; BINDING 118; /ligand="a ganglioside GT1b (d18:1(4E))"; /ligand_id="ChEBI:CHEBI:78452"; /evidence="ECO:0000250\|UniProtKB:P20917"; BINDING 124..128; /ligand="a ganglioside GT1b (d18:1(4E))"; /ligand_id="ChEBI:CHEBI:78452"; /evidence="ECO:0000250\|UniProtKB:P20917"
Subellular location Cell membrane ; Single-pass type I membrane protein. Membrane raft .
Tissues Both isoform 1 and isoform 2 are detected in myelinated structures in the central and peripheral nervous system, in periaxonal myelin and at Schmidt-Lanterman incisures (PubMed:6200494, PubMed:9495552). Detected in optic nerve, in oligodendroglia and in periaxonal myelin sheaths (PubMed:6200494). Detected in compact myelin (at protein level) (PubMed:6200494). Both isoform 1 and isoform 2 are detected in the central and peripheral nervous system (PubMed:9495552).
Structure Monomer and homodimer (By similarity). Interacts (via the first three N-terminal Ig-like domains) with RTN4R and RTN4RL2 (By similarity). Interacts with RTN4R (PubMed:19052207). Interacts with isoform 2 of BSG (By similarity).
Post-translational modification N-glycosylated.; Phosphorylated on tyrosine residues.; Ubiquitinated, leading to proteasomal degradation.
Target Relevance information above includes information from UniProt accession: P20916
The UniProt Consortium

ELISA with Biotinylated Human Siglec-4a/MAG Protein

Immobilized Anti-Siglec-4a Antibody, hFc Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human Siglec-4a, His Tag with the EC50 of 31.1ng/ml determined by ELISA.

HPLC of Biotinylated Human Siglec-4a/MAG Protein

The purity of Biotinylated Human Siglec-4a is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human Siglec-4a/MAG Protein

Biotinylated Human Siglec-4a on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.