SPR with Mouse Nectin-2/CD112 Protein 3581

ELISA with Mouse Nectin-2/CD112 Protein 3581

Mouse Nectin-2/CD112 Protein 3581

$240.00 – $800.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln32-Gly351

SKU: 3581parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P32507
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.
molecular weight	The protein has a predicted MW of 35.7 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse Nectin-2/CD112 Protein 3581

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.
Protein names Nectin-2 (Herpes virus entry mediator B) (Herpesvirus entry mediator B) (HveB) (Murine herpes virus entry protein B) (mHveB) (Nectin cell adhesion molecule 2) (Poliovirus receptor homolog) (Poliovirus receptor-related protein 2) (CD antigen CD112)
Gene names Nectin2,Nectin2 Mph Pvr Pvrl2 Pvs
Protein family Nectin family
Mass 10090Da
Function Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive. Probable cell adhesion protein.
Subellular location Cell membrane ; Single-pass type I membrane protein .
Tissues Brain, spinal cord, spleen, kidney, heart and liver.
Structure Can form trans-heterodimers with NECTIN3 (PubMed:10744716, PubMed:22902367). Interacts with CD226 or with PVRIG; these interactions are competitive and have a differential functional outcome on T-cell activation, either positive or negative, respectively. Binds with low affinity to TIGIT (By similarity).
Target Relevance information above includes information from UniProt accession: P32507
The UniProt Consortium

Mouse PVRIG, hFc Tag captured on CM5 Chip via Protein A can bind Mouse Nectin-2, His Tag with an affinity constant of 0.355 µM as determined in SPR assay (Biacore T200).

Immobilized Mouse Nectin-2, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Mouse PVRIG, hFc Tag with the EC50 of 0.81µg/ml determined by ELISA (QC Test).

The purity of Mouse Nectin-2 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Mouse Nectin-2/CD112 Protein

Mouse Nectin-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.