Human IL-33 Protein 3247

$345.00 – $1,150.00

Summary

Expression: E.coli
Functional: Yes (ELISA)
Amino Acid Range: Ser112-Thr270

SKU: 3247parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_254274
express system	E.coli
product tag	No Tag
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Interleukin-33 (IL-33) is a cytokine belonging to the IL-1 family, playing a role in inflammatory, infectious and autoimmune diseases and expressed in the cellular nucleus in several tissues. High levels of IL-33 are expressed in epithelial barrier tissues and endothelial barriers. ST2 is a receptor for IL-33, expressed selectively on a subset of Th2 cells, mediating some of their functions.
molecular weight	The protein has a predicted MW of 18 kDa same as Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human IL-33 Protein 3247

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Interleukin-33 (IL-33) is a cytokine belonging to the IL-1 family, playing a role in inflammatory, infectious and autoimmune diseases and expressed in the cellular nucleus in several tissues. High levels of IL-33 are expressed in epithelial barrier tissues and endothelial barriers. ST2 is a receptor for IL-33, expressed selectively on a subset of Th2 cells, mediating some of their functions.
Protein names Interleukin-33 (IL-33) (Interleukin-1 family member 11) (IL-1F11) (Nuclear factor from high endothelial venules) (NF-HEV) [Cleaved into: Interleukin-33 (95-270); Interleukin-33 (99-270); Interleukin-33 (109-270)]
Protein family IL-1 family
Mass 30759Da
Function Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016, PubMed:19841166). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines (PubMed:17853410, PubMed:18836528). Also involved in activation of mast cells, basophils, eosinophils and natural killer cells (PubMed:17853410, PubMed:18836528). Acts as an enhancer of polarization of alternatively activated macrophages (PubMed:19841166). Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528). Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity). {ECO:0000250\|UniProtKB:Q8BVZ5, ECO:0000269\|PubMed:16286016, ECO:0000269\|PubMed:17853410, ECO:0000269\|PubMed:18836528, ECO:0000269\|PubMed:19841166}.; In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or pro-inflammatory activation (PubMed:18787100). {ECO:0000269\|PubMed:18787100, ECO:0000269\|PubMed:21734074}.
Subellular location Nucleus {ECO:0000269\|PubMed:12819012, ECO:0000269\|PubMed:17185418, ECO:0000269\|PubMed:18787100, ECO:0000269\|PubMed:18836528, ECO:0000269\|PubMed:21734074}. Chromosome {ECO:0000269\|PubMed:17185418}. Cytoplasm {ECO:0000269\|PubMed:32272059}. Cytoplasmic vesicle, secretory vesicle {ECO:0000269\|PubMed:22215666}. Secreted {ECO:0000269\|PubMed:19465481, ECO:0000269\|PubMed:35794369}. Note=Secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore following cleavage by CELA1 (PubMed:35794369). Associates with heterochromatin and mitotic chromosomes (PubMed:17185418). The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion (PubMed:32272059). {ECO:0000269\|PubMed:32272059, ECO:0000269\|PubMed:35794369}.
Tissues Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta. {ECO:0000269\|PubMed:12819012}.
Structure Forms a 1:1:1 heterotrimeric complex with its primary high-affinity receptor IL1RL1 and the coreceptor IL1RAP (PubMed:19836339, PubMed:23980170). Interacts with cargo receptor TMED10; the interaction mediates the translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and thereby secretion (PubMed:32272059). {ECO:0000269\|PubMed:19836339, ECO:0000269\|PubMed:23980170, ECO:0000269\|PubMed:32272059}.; (Microbial infection) Interacts (in reduced form) with H.polygyrus ARI. {ECO:0000269\|PubMed:29045903}.
Post-translational modification The full-length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CELA1, CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein (PubMed:22307629, PubMed:35794369). May also be proteolytically processed by calpains (PubMed:19596270, PubMed:22307629). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016, PubMed:19439663) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase (PubMed:19439663, PubMed:19465481). {ECO:0000269\|PubMed:16286016, ECO:0000269\|PubMed:19439663, ECO:0000269\|PubMed:19465481, ECO:0000269\|PubMed:19559631, ECO:0000269\|PubMed:19596270, ECO:0000269\|PubMed:22307629, ECO:0000269\|PubMed:35794369}.
Domain The homeodomain-like HTH domain mediates nuclear localization and heterochromatin associatio
Target Relevance information above includes information from UniProt accession: O95760
The UniProt Consortium

Immobilized Human IL-33, No Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human IL-1RL1, His Tag with the EC50 of 9.6ng/ml determined by ELISA.

The purity of Human IL-33 is greater than 95% as determined by SEC-HPLC.

Human IL-33 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.