Human NGAL/Lipocalin-2 Protein 3095

$203.00 – $675.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln21-Gly198

SKU: 3095parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P80188
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Acute kidney injury (AKI) is one of the most common complications of various serious conditions, and early diagnosis is therefore critical for the treatment of AKI. Recent evidence demonstrates that neutrophil gelatinase- associated lipocalin (NGAL) is closely associated with AKI. Several experimental and clinical studies have shown that the expression of urine and serum NGAL increases significantly in AKI. NGAL shows potential to be a new effective early biochemical marker of AKI. Further studies are needed to confirm the significant advantages of NGAL in the diagnosis of early AKI and its value in clinical applications.
molecular weight	The protein has a predicted MW of 21.64 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human NGAL/Lipocalin-2 Protein 3095

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Acute kidney injury (AKI) is one of the most common complications of various serious conditions, and early diagnosis is therefore critical for the treatment of AKI. Recent evidence demonstrates that neutrophil gelatinase- associated lipocalin (NGAL) is closely associated with AKI. Several experimental and clinical studies have shown that the expression of urine and serum NGAL increases significantly in AKI. NGAL shows potential to be a new effective early biochemical marker of AKI. Further studies are needed to confirm the significant advantages of NGAL in the diagnosis of early AKI and its value in clinical applications.
Protein names Neutrophil gelatinase-associated lipocalin (NGAL) (25 kDa alpha-2-microglobulin-related subunit of MMP-9) (Lipocalin-2) (Oncogene 24p3) (Siderocalin) (p25)
Protein family Calycin superfamily, Lipocalin family
Mass 22588Da
Function Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed:12453413, PubMed:20581821, PubMed:27780864). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis (By similarity). Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed:27780864). Can also bind siderophores from M.tuberculosis (PubMed:15642259, PubMed:21978368). {ECO:0000250\|UniProtKB:P11672, ECO:0000269\|PubMed:12453413, ECO:0000269\|PubMed:15642259, ECO:0000269\|PubMed:20581821, ECO:0000269\|PubMed:21978368, ECO:0000269\|PubMed:27780864}.
Subellular location Secreted {ECO:0000269\|PubMed:12453413, ECO:0000269\|PubMed:27780864, ECO:0000269\|PubMed:7683678}. Cytoplasmic granule lumen {ECO:0000269\|PubMed:8298140}. Cytoplasmic vesicle lumen {ECO:0000269\|PubMed:12453413}. Note=Upon binding to the SLC22A17 (24p3R) receptor, it is internalized (By similarity). Releases the bound iron in the acidic lumen of cytoplasmic vesicles (PubMed:12453413, PubMed:20581821). {ECO:0000250\|UniProtKB:P11672, ECO:0000269\|PubMed:12453413, ECO:0000269\|PubMed:20581821}.
Tissues Detected in neutrophils (at protein level) (PubMed:7683678, PubMed:8298140). Expressed in bone marrow and in tissues that are prone to exposure to microorganism (PubMed:9339356). High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung (PubMed:9339356). Expressed in the medullary tubules of the kidney (PubMed:30418175). Not found in the small intestine or peripheral blood leukocytes (PubMed:9339356). {ECO:0000269\|PubMed:30418175, ECO:0000269\|PubMed:7683678, ECO:0000269\|PubMed:8298140, ECO:0000269\|PubMed:9339356}.
Structure Monomer (PubMed:1281792, PubMed:7683678). Homodimer; disulfide-linked (PubMed:7683678). Heterodimer; disulfide-linked with MMP9 (PubMed:7683678). {ECO:0000269\|PubMed:12453412, ECO:0000269\|PubMed:1281792, ECO:0000269\|PubMed:7683678}.
Target Relevance information above includes information from UniProt accession: P80188
The UniProt Consortium

Data

ELISA with Human NGAL/Lipocalin-2 Protein

Immobilized Human NGAL, His Tag at 0.2µg/ml (100µl/Well) on the plate. Dose response curve for Anti-NGAL Antibody, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA.

The purity of Human NGAL is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human NGAL/Lipocalin-2 Protein

Human NGAL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.