Human IL-2 R beta/CD122 Protein 3032

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ala27-Asp239

SKU: 3032parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_000869
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains.
molecular weight	The protein has a predicted MW of 51.35 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human IL-2 R beta/CD122 Protein 3032

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

No information

target relevance

Data

SPR with Human IL-2 R beta/CD122 Protein

Human IL-2 R beta, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.216 µM as determined in SPR assay (Biacore T200).

Human IL-2 R beta, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15, No Tag with an affinity constant of 2.88 nM as determined in SPR assay (Biacore T200).

ELISA with Human IL-2 R beta/CD122 Protein

Immobilized Human IL-2 R beta, hFc Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human IL-15Ra&IL-15, His Tag with the EC50 of 18.5ng/ml determined by ELISA (QC Test).

The purity of Human IL-2 R beta is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human IL-2 R beta/CD122 Protein

Human IL-2 R beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.