Cynomolgus uPAR/PLAUR Protein 5081

$225.00 – $750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Leu23-Gly305

SKU: 5081parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q9GK78
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR.
molecular weight	The protein has a predicted MW of 32.69 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus uPAR/PLAUR Protein 5081

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR.
Protein names Urokinase plasminogen activator surface receptor (U-PAR) (uPAR) (CD antigen CD87)
Gene names PLAUR,PLAUR UPAR
Mass 9541Da
Function Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form (By similarity).
Subellular location Cell membrane. Cell projection, invadopodium membrane. Cell membrane ; Lipid-anchor, GPI-anchor. Note=Colocalized with FAP (seprase) preferentially at the cell surface of invadopodia membrane in a cytoskeleton-, integrin- and vitronectin-dependent manner.
Structure Monomer (Probable). Interacts (via the UPAR/Ly6 domains) with SRPX2. Interacts with MRC2. Interacts with FAP (seprase); the interaction occurs at the cell surface of invadopodia membrane. Interacts with SORL1 (via N-terminal ectodomain); this interaction decreases PLAUR internalization (By similarity). The ternary complex composed of PLAUR-PLAU-SERPINE1 also interacts with SORL1 (By similarity).
Target Relevance information above includes information from UniProt accession: Q9GK78
The UniProt Consortium

Data

Cynomolgus uPAR, His Tag immobilized on CM5 Chip can bind Cynomolgus PLAU, His Tag with an affinity constant of 0.033 nM as determined in SPR assay (Biacore T200).

ELISA with Cynomolgus uPAR/PLAUR Protein

Immobilized Cynomolgus uPAR, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test).

The purity of Cynomolgus uPAR is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus uPAR/PLAUR Protein

Cynomolgus uPAR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.