Human CD96/TACTILE (C110S) Protein 2945

$315.00 – $1,050.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Val22-Met503(C110S)

available size

Clear

SKU: 2945parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P40200
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The receptors CD96 and TIGIT are expressed on the surface of T and natural killer (NK) cells, and recent studies suggest both play important inhibitory roles in immune function. CD96 has been shown to modulate immune cell activity in mice, with Cd96-/- mice displaying hypersensitive NK-cell responses to immune challenge and significant tumor resistance. The counterbalance between the putative inhibitory CD96 and TIGIT receptors and the activating receptor, CD226, offers unique strategies for immuno-oncology drug development.
molecular weight	The protein has a predicted MW of 54.63 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human CD96/TACTILE (C110S) Protein 2945

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The receptors CD96 and TIGIT are expressed on the surface of T and natural killer (NK) cells, and recent studies suggest both play important inhibitory roles in immune function. CD96 has been shown to modulate immune cell activity in mice, with Cd96-/- mice displaying hypersensitive NK-cell responses to immune challenge and significant tumor resistance. The counterbalance between the putative inhibitory CD96 and TIGIT receptors and the activating receptor, CD226, offers unique strategies for immuno-oncology drug development.
Protein names T-cell surface protein tactile (Cell surface antigen CD96) (T cell-activated increased late expression protein) (CD antigen CD96)
Gene names CD96,CD96
Mass 9606Da
Function May be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation.
Catalytic activity #N/A
Subellular location Membrane; Single-pass type I membrane protein.
Tissues Expressed on normal T-cell lines and clones, and some transformed T-cells, but no other cultured cell lines tested. It is expressed at very low levels on activated B-cells.
Structure Homodimer; disulfide-linked. Interacts with PVR.
Target Relevance information above includes information from UniProt accession: P40200
The UniProt Consortium

Data

SPR with Human CD96/TACTILE (C110S) Protein

Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Human CD96 (C110S) , His Tag with an affinity constant of 0.289 µM as determined in SPR assay (Biacore T200).

HPLC of Human CD96/TACTILE (C110S) Protein

The purity of Human CD96 (C110S) is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human CD96/TACTILE (C110S) Protein

Human CD96 (C110S) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.