Cynomolgus RGM-C Protein 2675

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln36-Asp401

SKU: 2675parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	XP_005542141
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	RGM gene family ('Repulsive Axonal Guidance molecules' A, B and C), both RGM A and B are mostly expressed in central nervous system, while RGM C is exclusively expressed in all striated muscle and in the myocardium. RGM A and B appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGM A appears in the neuroepithelium, and only RGM B in the peripheral nervous system.
molecular weight	The protein has a predicted MW of 40 kDa (mature)&26 kDa (C-terminus peptide)&14 kDa (N-terminus peptide). Due to enzyme lysis and glycosylation, the protein migrates to 50-60 kDa&30-40 kDa&20-25 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus RGM-C Protein 2675

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
RGM gene family ('Repulsive Axonal Guidance molecules' A, B and C),both RGM A and B are mostly expressed in central nervous system, while RGM C is exclusively expressed in all striated muscle and in the myocardium. RGM A and B appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGM A appears in the neuroepithelium, and only RGM B in the peripheral nervous system.
Protein names Hemojuvelin (Hemochromatosis type 2 protein) (Hemojuvelin BMP coreceptor) (RGM domain family member C)
Protein family Repulsive guidance molecule (RGM) family
Mass 45080Da
Function Acts as a bone morphogenetic protein (BMP) coreceptor (PubMed:18976966). Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis (PubMed:18976966). {ECO:0000269\|PubMed:18976966}.
Subellular location Cell membrane {ECO:0000250}; Lipid-anchor, GPI-anchor {ECO:0000250}. Note=Also released in the extracellular space. {ECO:0000269\|PubMed:25156943}.
Tissues Adult and fetal liver, heart, and skeletal muscle. {ECO:0000269\|PubMed:14647275}.
Structure Interacts with BMP2 and BMP4 (By similarity). Interacts with BMP6 (By similarity). Interacts with BMPR1B (PubMed:16604073). Interacts with TMPRSS6 (PubMed:18976966, PubMed:19357398). {ECO:0000250\|UniProtKB:Q7TQ32, ECO:0000269\|PubMed:16604073, ECO:0000269\|PubMed:18976966, ECO:0000269\|PubMed:19357398}.
Post-translational modification Autocatalytically cleaved at low pH; the two chains remain linked via two disulfide bonds (PubMed:25156943). Also proteolytically processed by TMPRSS6, several fragments being released in the extracellular space; regulates HJV activity in BMP signaling and thefore iron homeostasis (PubMed:18976966, PubMed:25156943). {ECO:0000269\|PubMed:18976966, ECO:0000269\|PubMed:25156943}.
Target Relevance information above includes information from UniProt accession: Q6ZVN8
The UniProt Consortium

Data

Immobilized Cynomolgus RGM-C, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-RGM-C Antibody, hFc Tag with the EC50 of 1.7ng/ml determined by ELISA.

The purity of Cynomolgus RGM-C is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus RGM-C Protein

Cynomolgus RGM-C on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.