Human DKK1 C terminal Domain Protein 2584

$1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Met178-His266

SKU: 2584parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	O94907
express system	HEK293
product tag	C-mFc
purity	> 95% as determined by Tris-Bis PAGE
background	Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.
molecular weight	The protein has a predicted MW of 35.78 kDa. Due to glycosylation, the protein migrates to 45-53 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human DKK1 C terminal Domain Protein 2584

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.
Protein names Dickkopf-related protein 1 (Dickkopf-1) (Dkk-1) (hDkk-1) (SK)
Gene names DKK1,DKK1 UNQ492/PRO1008
Protein family Dickkopf family
Mass 9606Da
Function Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed:22000856). DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (PubMed:17143291). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (By similarity).
Subellular location Secreted.
Tissues Placenta.
Structure Interacts with LRP6 (PubMed:11448771, PubMed:17804805, PubMed:20093360, PubMed:22000856). Interacts (via the C-termianl Cys-rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt-mediated signaling (PubMed:19746449). Forms a ternary complex with LRP6 and KREM1 (PubMed:27524201). Interacts with KREM1 (PubMed:17804805).
Domain Th
Target Relevance information above includes information from UniProt accession: O94907
The UniProt Consortium

Data

ELISA with Human DKK1 C terminal Domain Protein

Immobilized Human DKK1 C terminal Domain, mFc Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA.

SDS-PAGE gel of Human DKK1 C terminal Domain Protein

Human DKK1 C terminal Domain on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.