Human Claudin 4 Protein-VLP 2456

$1,100.00 – $3,800.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Met1-Val209

SKU: 2456parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	O14493
express system	HEK293
product tag
purity	> 95% as determined by HPLC
background	Claudin-4 (CLDN4) is a key component of tight junctions (TJs) in epithelial cells. CLDN4 is overexpressed in many epithelial malignancies and correlates with cancer progression. Changes in CLDN4 expression have been associated with epigenetic factors (such as hypomethylation of promoter DNA), inflammation associated with infection and cytokines, and growth factor signaling. CLDN4 helps to maintain the tumor microenvironment by forming TJs and acts as a barrier to the entry of anticancer drugs into tumors.
molecular weight	The target protein has a predicted MW of 23.31 kDa.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human Claudin 4 Protein-VLP 2456

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Claudin-4 (CLDN4) is a key component of tight junctions (TJs) in epithelial cells. CLDN4 is overexpressed in many epithelial malignancies and correlates with cancer progression. Changes in CLDN4 expression have been associated with epigenetic factors (such as hypomethylation of promoter DNA), inflammation associated with infection and cytokines, and growth factor signaling. CLDN4 helps to maintain the tumor microenvironment by forming TJs and acts as a barrier to the entry of anticancer drugs into tumors.
Protein names Claudin-4 (Clostridium perfringens enterotoxin receptor) (CPE-R) (CPE-receptor) (Williams-Beuren syndrome chromosomal region 8 protein)
Gene names CLDN4,CLDN4 CPER CPETR1 WBSCR8
Protein family Claudin family
Mass 9606Da
Function Channel-forming tight junction protein that mediates paracellular chloride transport in the kidney. Plays a critical role in the paracellular reabsorption of filtered chloride in the kidney collecting ducts. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Subellular location Cell junction, tight junction. Cell membrane ; Multi-pass membrane protein. Note=CLDN4 is required for tight junction localization in the kidney.
Structure Directly interacts with TJP1/ZO-1 (PubMed:16236711). Interacts with TJP2/ZO-2 and TJP3/ZO-3 (By similarity). Interacts with EPHA2; phosphorylates CLDN4 and may regulate tight junctions (PubMed:16236711). Interacts with CLDN1 (PubMed:20375010). Interacts with CLDN8 (By similarity).; (Microbial infection) Interacts (via both extracellular domains) with Clostridium perfringens enterotoxin CPE; the interaction may disrupt claudin assembly in tight junctions.
Post-translational modification Phosphorylated. Phosphorylation by EPHA2 is stimulated by EFNA1 and alters interaction with TJP1.
Target Relevance information above includes information from UniProt accession: O14493
The UniProt Consortium

Data

Immobilized Human Claudin 4 VLP at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-Claudin 4 Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA.

The purity of Human Claudin 4 VLP is greater than 95% as determined by SEC-HPLC.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.