Mouse BID Protein 2433

$345.00 – $1,150.00

Summary

Expression: E.coli
Binding assay: Yes (SPR)
Amino Acid Range: Ala1-Ser195

SKU: 2433parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	EDK99650
express system	E.coli
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
background	Bid, BH3-interacting domain death agonist, was initially cloned based in its ability to interact with both Bcl-2 and Bax. Bid contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. Bid is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Bid is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals.
molecular weight	The protein has a predicted MW of 22.72 kDa same as Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse BID Protein 2433

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Bid, BH3-interacting domain death agonist, was initially cloned based in its ability to interact with both Bcl-2 and Bax. Bid contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. Bid is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Bid is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals.
Protein names BH3-interacting domain death agonist (p22 BID) (BID) [Cleaved into: BH3-interacting domain death agonist p15 (p15 BID); BH3-interacting domain death agonist p13 (p13 BID); BH3-interacting domain death agonist p11 (p11 BID)]
Mass 21995Da
Function Induces caspases and apoptosis (PubMed:14583606). Counters the protective effect of BCL2 (By similarity). {ECO:0000250\|UniProtKB:P70444, ECO:0000269\|PubMed:14583606}.; [BH3-interacting domain death agonist p15]: Induces caspase activation and apoptosis (PubMed:15661737, PubMed:32029622). Allows the release of cytochrome c (PubMed:32029622). {ECO:0000269\|PubMed:15661737, ECO:0000269\|PubMed:32029622}.; [Isoform 1]: Induces ICE-like proteases and apoptosis. {ECO:0000269\|PubMed:14583606}.; [Isoform 2]: Induces ICE-like proteases and apoptosis. {ECO:0000269\|PubMed:14583606}.; [Isoform 3]: Does not induce apoptosis. {ECO:0000269\|PubMed:14583606}.; [Isoform 4]: Induces ICE-like proteases and apoptosis. {ECO:0000269\|PubMed:14583606}.
Subellular location Cytoplasm {ECO:0000269\|PubMed:14583606}. Mitochondrion membrane {ECO:0000269\|PubMed:14583606}. Mitochondrion outer membrane {ECO:0000269\|PubMed:29531808}. Note=When uncleaved, it is predominantly cytoplasmic. {ECO:0000269\|PubMed:14583606}.; [BH3-interacting domain death agonist p15]: Mitochondrion membrane {ECO:0000250\|UniProtKB:P70444}. Note=Translocates to mitochondria as an integral membrane protein. {ECO:0000250\|UniProtKB:P70444}.; [BH3-interacting domain death agonist p13]: Mitochondrion membrane {ECO:0000250\|UniProtKB:P70444}. Note=Associated with the mitochondrial membrane. {ECO:0000250\|UniProtKB:P70444}.; [Isoform 1]: Cytoplasm {ECO:0000269\|PubMed:14583606}.; [Isoform 3]: Cytoplasm {ECO:0000269\|PubMed:14583606}.; [Isoform 2]: Mitochondrion membrane {ECO:0000269\|PubMed:14583606}. Note=A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively. {ECO:0000269\|PubMed:14583606}.
Tissues [Isoform 2]: Expressed in spleen, pancreas and placenta (at protein level). {ECO:0000269\|PubMed:14583606}.; [Isoform 3]: Expressed in lung, pancreas and spleen (at protein level). {ECO:0000269\|PubMed:14583606}.; [Isoform 4]: Expressed in lung and pancreas (at protein level). {ECO:0000269\|PubMed:14583606}.
Structure Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2 (By similarity). Interacts with PLEKHN1 (PubMed:29531808). {ECO:0000250\|UniProtKB:P70444, ECO:0000269\|PubMed:29531808}.; [BH3-interacting domain death agonist]: Interacts with humanin; forms fibers with humanin which results in BID conformational changes and sequestering of BID into the fibers, preventing BID activation. {ECO:0000269\|PubMed:15661737, ECO:0000269\|PubMed:33106313}.; [BH3-interacting domain death agonist p15]: Interacts with ITCH (PubMed:20392206). Interacts with humanin; the interaction prevents BID-induced apoptosis (PubMed:15661737). Interacts with MTCH2 (By similarity). {ECO:0000250\|UniProtKB:P70444, ECO:0000269\|PubMed:15661737, ECO:0000269\|PubMed:20392206}.
Post-translational modification [BH3-interacting domain death agonist]: TNF-alpha induces caspase-mediated cleavage into a major p15 and minor p13 and p11 products (By similarity). Cleaved by CASP6 into a major p15 and minor p13 products, leading to release of cytochrome c and subsequent nonalcoholic steatohepatitis (PubMed:32029622). {ECO:0000250\|UniProtKB:P70444, ECO:0000269\|PubMed:32029622}.; [BH3-interacting domain death agonist p15]: Ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation. {ECO:0000269\|PubMed:20392206}.
Domain Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity
Target Relevance information above includes information from UniProt accession: P55957
The UniProt Consortium

Data

Mouse BID, His Tag immobilized on CM5 Chip can bind Human BCL2L1, His Tag with an affinity constant of 33.06 nM as determined in SPR assay (Biacore T200).

The purity of Mouse BID is greater than 90% as determined by SEC-HPLC.

Mouse BID on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.