Canine BMPR1A/ALK-3 Protein 2425

$345.00 – $1,150.00

Summary

Expression: HEK293
Active: Yes (cell based assay)
Amino Acid Range: Gln24-Arg152

SKU: 2425parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_001138622
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The type IA bone morphogenetic protein receptor (Bmpr1a), encoded by 11 exons and spanning about 40 kb on chromosome 14 in mice and chromosome 10 in human (Derynck & Feng, 1997; Mishina, Hanks, Miura, Tallquist, & Behringer, 2002), is an essential receptor for BMP signaling.
molecular weight	The protein has a predicted MW of 40.94 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Canine BMPR1A/ALK-3 Protein 2425

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
The type IA bone morphogenetic protein receptor (Bmpr1a), encoded by 11 exons and spanning about 40 kb on chromosome 14 in mice and chromosome 10 in human (Derynck & Feng, 1997; Mishina, Hanks, Miura, Tallquist, & Behringer, 2002), is an essential receptor for BMP signaling.
Protein names
Bone morphogenetic protein receptor type-1A (BMP type-1A receptor) (BMPR-1A) (EC 2.7.11.30) (Activin receptor-like kinase 3) (ALK-3) (Serine/threonine-protein kinase receptor R5) (SKR5) (CD antigen CD292)
Protein family
Protein kinase superfamily, TKL Ser/Thr protein kinase family, TGFB receptor subfam
Mass
60198Da
Function
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP2, BMP4, GDF5 and GDF6. Positively regulates chondrocyte differentiation through GDF5 interaction. Mediates induction of adipogenesis by GDF6. May promote the expression of HAMP, potentially via its interaction with BMP2 (By similarity). {ECO:0000250|UniProtKB:P36895}.
Catalytic activity
CATALYTIC ACTIVITY: Reaction=ATP + L-threonyl-[receptor-protein] = ADP + H(+) + O-phospho-L-threonyl-[receptor-protein]; Xref=Rhea:RHEA:44880, Rhea:RHEA-COMP:11024, Rhea:RHEA-COMP:11025, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.30; CATALYTIC ACTIVITY: Reaction=ATP + L-seryl-[receptor-protein] = ADP + H(+) + O-phospho-L-seryl-[receptor-protein]; Xref=Rhea:RHEA:18673, Rhea:RHEA-COMP:11022, Rhea:RHEA-COMP:11023, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.30;
Subellular location
Cell membrane {ECO:0000250|UniProtKB:P36898}; Single-pass type I membrane protein {ECO:0000255}. Cell surface {ECO:0000250|UniProtKB:P36895}.
Tissues
Highly expressed in skeletal muscle.
Structure
Interacts with low affinity with GDF5; positively regulates chondrocyte differentiation (PubMed:24098149). Interacts with BMP4 (PubMed:8006002). Interacts with SCUBE3 (PubMed:33308444). Interacts with TSC22D1/TSC-22 (PubMed:21791611). Interacts with BMP2; the interaction may induce HAMP expression (PubMed:10881198, PubMed:15064755, PubMed:17295905, PubMed:18937504, PubMed:31800957). Interacts with BMP6 (PubMed:31800957). Interacts with heterodimers composed of BMP2 and BMP6 in vitro; the interaction may induce HAMP expression (PubMed:31800957). {ECO:0000269|PubMed:10881198, ECO:0000269|PubMed:15064755, ECO:0000269|PubMed:17295905, ECO:0000269|PubMed:18937504, ECO:0000269|PubMed:21791611, ECO:0000269|PubMed:24098149, ECO:0000269|PubMed:31800957, ECO:0000269|PubMed:33308444, ECO:0000269|PubMed:8006002}.
Post-translational modification
Glycosylated. {ECO:0000250|UniProtKB:P36895}.
Target Relevance information above includes information from UniProt accession: P36894
The UniProt Consortium

Data

Cell based assay with Canine BMPR1A/ALK-3 Protein

Measured by its ability to inhibit rhBMP4-induced alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is typically 0.5 - 2.5 µg/ml in the presence of 50 ng/mL of recombinant human BMP4.

The purity of Canine BMPR1A is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Canine BMPR1A/ALK-3 Protein

Canine BMPR1A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.