SDS-PAGE gel of Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A4 (KVLEHVV 2055

Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A4 (KVLEHVVRV) Monomer Protein 2055

$310.00 – $1,125.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and KVLEHVVRV peptide

available size

Clear

SKU: 2055parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse HLd α3)&&P01887(Mouse B2M)&KVLEHVVRV
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	MAGE-A4 and MAGE-A8 are type I membes of the melanoma associated antigen (MAGE) family. The MAGE family is a large, highly conserved group of proteins that share a common MAGE homology domain. Both MAGE-A4 and MAGE-A8 antigen-presenting peptides can be presented by HLA-A*02:01.
molecular weight	The protein has a predicted MW of 48.00 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A4 (KVLEHVVRV) Monomer Protein 2055

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
MAGE-A4 and MAGE-A8 are type I membes of the melanoma associated antigen (MAGE) family. The MAGE family is a large, highly conserved group of proteins that share a common MAGE homology domain. Both MAGE-A4 and MAGE-A8 antigen-presenting peptides can be presented by HLA-A*02:01.
Protein names Major histocompatibility complex, class I, A
Gene names HLA-A,HLA-A
Protein family MHC class I family
Mass 41449Da
Target Relevance information above includes information from UniProt accession: A0A140T913
The UniProt Consortium

Data

SPR with Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A4 (KVLEHVVRV) Mon

HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) TCR immobilized on CM5 Chip can bind Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A4 (KVLEHVVRV) Monomer, His Tag with an affinity constant of 4.76 µM as determined in SPR assay (Biacore T200).

HPLC of Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A4 (KVLEHVVRV) Mono

The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A4 (KVLEHVVRV) Monomer is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A4 (KVLEHVV

Chimeric HLA-A*02:01 (mα3) &mB2M&MAGE-A4 (KVLEHVVRV) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.