| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | T7 |
| available sizes | 1 mg |
goat anti-T7 HRP polyclonal antibody 5237
$3,142.00
Antibody summary
- Goat polyclonal to T7 , HRP conjugate
- Suitable for: WB,IHC,ICC,ELISA
- Isotype: Whole IgG
- 1 mg
goat anti-T7 HRP polyclonal antibody 5237
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000- 1:30,000. Immunocytochemistry: use at a dilution of 1:200-1:500 ELISA: use at a dilution of 1:10,000- 1:100,000 These are recommended dilutions. Endusers should determine optimal dilutions for their applications. |
| Immunogen T7 (MASMTGGQQMG) conjugated to KLH. |
| Size and concentration 1mg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer Phosphate Buffered Saline (PBS) containing 0.2% |
| Purity peptide affinty purifcation |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705 |
| Isotype control Goat polyclonal - Isotype Control |
| target relevance |
|---|
| Biotechnology The T7 tag has emerged as a valuable tool in scientific research, particularly in molecular biology and protein expression studies. The T7 tag is a short peptide sequence (MASMTGGQQMG) derived from the T7 bacteriophage gene 10, which can be fused to the N or C terminus of a target protein. Its small size and minimal impact on the function of the tagged protein make it an attractive choice as a molecular tag. Researchers frequently use the T7 tag in protein expression systems, such as bacterial, insect, or mammalian cells, to facilitate the identification and purification of the recombinant protein. The T7 tag???s versatility allows for straightforward detection using specific T7 tag antibodies in various experimental assays, including Western blotting and immunoprecipitation. Moreover, the T7 tag can be employed for protein purification through affinity chromatography using T7 tag-specific resins, simplifying downstream characterization and functional studies. The widespread adoption of the T7 tag in research owes to its efficiency, reproducibility, and compatibility with a range of expression systems, making it an indispensable tool for investigating protein interactions, post-translational modifications, and protein localization in diverse biological contexts. |
 |
| Detection of T7-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, goat anti-T7 antibody 5237 used for WB at 0.2 µg /ml (1:5,000). Detection: Chemiluminescence with an exposure time of 30 seconds. |
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
| relevant to this product |
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| Western blot IHC ICC |
| # | SDS | Certificate | |
|---|---|---|---|
| Please enter your product and batch number here to retrieve product datasheet, SDS, and QC information. | |||
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