Human ENPP-2/Autotaxin Protein 4789

$315.00 – $1,050.00

Summary

Expression: HEK293
Active: Yes (catalytic)
Amino Acid Range: Asp49-Ile863

SKU: 4789parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_001035181
express system	HEK293
product tag	N-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Ectonucleotide pyrophosphatase/phosphodiesterase 2 (ENPP2) also known as Autotaxin, is a secreted lysophospholipase D, which hydrolyzes lysophosphatidylcholine (LPC) into Lysophosphatidic acid (LPA). ENPP2 is an essential protein for normal development and its altered expression is associated with various human diseases.
molecular weight	The protein has a predicted MW of 94.8 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human ENPP-2/Autotaxin Protein 4789

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Ectonucleotide pyrophosphatase/phosphodiesterase 2 (ENPP2) also known as Autotaxin, is a secreted lysophospholipase D, which hydrolyzes lysophosphatidylcholine (LPC) into Lysophosphatidic acid (LPA). ENPP2 is an essential protein for normal development and its altered expression is associated with various human diseases.
Protein names Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (E-NPP 2) (EC 3.1.4.39) (Autotaxin) (Extracellular lysophospholipase D) (LysoPLD)
Gene names Enpp2,Enpp2 Npps2 Pdnp2
Protein family Nucleotide pyrophosphatase/phosphodiesterase family
Mass 10090Da
Function Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:17208043, PubMed:27780639, PubMed:28414242). Major substrate is lysophosphatidylcholine (PubMed:17208043, PubMed:27780639). Can also act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP (PubMed:18175805). Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition (By similarity). Possible involvement in cell proliferation and adipose tissue development (Probable). Tumor cell motility-stimulating factor. Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids (By similarity). Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) (By similarity).
Subellular location Secreted .
Tissues Expressed in brain and adipose tissue.
Post-translational modification N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity. Secretion requires simultaneous glycosylation on Asn-53 and Asn-410, while probable glycosylation of Asn-410 has a preferential role on lysoPLD activity. Not O-glycosylated.; The interdomain disulfide bond between Cys-413 and Cys-805 is essential for catalytic activity.
Target Relevance information above includes information from UniProt accession: Q9R1E6
The UniProt Consortium

Data

Activity assay with Human ENPP-2/Autotaxin Protein

Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP). The specific activity is > 10000 pmol/min/µg, as measured under the described conditions.

The purity of Human ENPP-2 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human ENPP-2/Autotaxin Protein

Human ENPP-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.