| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| target | Entero |
| species reactivity | For the detection of enterovirus (Coxsackievirus A, Coxsackievirus B, Echovirus, Poliovirus). |
| applications | RT PCR |
| assay type | direct & qualitative |
| available sizes | 96 tests |
Entero RT-PCR test Mikrogen 830509
$487.00
Summary
- Mikrogen diagnostik RT PCR kit for research use (RUO)
- Direct Coxsackievirus, Echovirus, Poliovirus, Enterovirus detection
- High sensitivity and specificity
- Internal control for monitoring nucleic acid extraction
(RNA/DNA) and real-time PCR inhibition in each reaction - Compatible with most common real-time PCR cyclers & RNA/DNA extraction methods
- 96 tests
Entero RT-PCR test Mikrogen 830509
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| Assay type RT PCR | |||||||||||||||
| Research area Infectious Disease | |||||||||||||||
| Sample type whole blood, serum, plasma, urine, tissue, stool, etc., food and environmental samples or from the carrier material | |||||||||||||||
Notes
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Components
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| Storage Store at -20°C. | |||||||||||||||
| Additional information Highly sensitive and specific direct detection of pathogens that can cause tick-borne infections
Applicable to human starting material as well as RNA/DNA from the tick |
| target relevance |
|---|
| Coxsackievirus |
| Description Virus that causes hand, foot and mouth disease, herpangina, myocarditis, and other enteroviral infections |
| Structure Coxsackieviruses are non-enveloped, positive-sense, single-stranded RNA viruses belonging to the genus Enterovirus within the family Picornaviridae. The viruses possess an icosahedral capsid that protects the viral RNA genome and contributes to environmental stability. Coxsackieviruses are divided into Group A and Group B based on their pathogenic properties and biological characteristics. These viruses are transmitted primarily through the fecal-oral route, respiratory secretions, and direct contact with contaminated surfaces. Following infection, viral replication typically occurs in the gastrointestinal and respiratory tracts before dissemination to other tissues. Coxsackieviruses are distributed worldwide and are a common cause of childhood and adult infections, with outbreaks occurring periodically in communities, schools, and daycare settings. |
| Diagnosis The diagnosis of Coxsackievirus infection is performed through molecular, virological, and serological methods. Reverse transcription polymerase chain reaction (RT-PCR) is the preferred diagnostic technique for detecting viral RNA in throat swabs, stool samples, cerebrospinal fluid, blood, and tissue specimens due to its high sensitivity and specificity. Viral culture may be used in specialized laboratories but has largely been replaced by molecular methods. Serological assays, including ELISA-based tests, can detect virus-specific antibodies and may be useful in epidemiological investigations or retrospective diagnosis. Clinical presentation and epidemiological context often play an important role in guiding diagnostic testing. |
| Symptoms Coxsackievirus infections produce a wide spectrum of clinical manifestations ranging from mild febrile illness to severe systemic disease. Group A coxsackieviruses commonly cause hand, foot and mouth disease and herpangina, characterized by fever, sore throat, painful oral ulcers, and vesicular lesions on the hands and feet. Group B coxsackieviruses are frequently associated with pleurodynia, myocarditis, pericarditis, and viral meningitis. Additional symptoms may include headache, fatigue, muscle aches, abdominal pain, nausea, vomiting, and rash. While most infections are self-limiting and resolve without complications, severe disease can occur in neonates, immunocompromised individuals, and patients with cardiac involvement, potentially resulting in life-threatening complications. |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What types of samples can be used with the Entero RT-PCR test Mikrogen 830509?
The Entero RT-PCR test Mikrogen 830509 can be used with a variety of sample types including whole blood, serum, plasma, urine, tissue, stool, as well as food and environmental samples or carrier material.
Is the Entero RT-PCR test Mikrogen 830509 compatible with common real-time PCR cyclers and RNA/DNA extraction methods?
Yes, the kit is compatible with most common real-time PCR cyclers such as Roche LightCycler 480 Instrument II, Roche cobas z 480 Analyzer, Qiagen Rotor-Gene Q, Bio-Rad CFX 96, Applied Biosystems QuantStudio 5 Dx, and Stratagene Mx3000P. It also supports various RNA/DNA extraction methods including Mikrogen alphaClean Mag RNA/DNA Kits.
Publications
| pmid | title | authors | citation |
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| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
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| This product has moved to a digital protocol. Please use the URL provided on the product packaging to access the electronic Instructions for Use (eIFU). 830509 protocol |
Documents
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