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Brucella IgA ELISA Kit ESR116A

$334.00

Summary

  • Virion/Serion Diagnostic Kit for research use (RUO)
  • Brucella IgA ELISA Kit
  • Suitable for IgA detection
  • Ready-to-use
  • 96 tests
Weight1 lbs
Dimensions9 × 5 × 2 in
target

Brucella reactive IgA

species reactivity

Brucella

applications

ELISA

assay type

Indirect & quantitative

available sizes

96 tests

Brucella IgA ELISA Kit ESR116A

kit
Assay type
Indirect ELISA
Research area
Infectious Disease
Sample type
Serum, plasma, whole blood
Notes
Pretreatment of samples with RF-Absorbent (Z200) is recommended for use with IgM ELISA kits to eliminate presence of sample rheumatoid factors and possible false negative results.
Components
Break apart microtiter test strips each with antigen coated single wells8 x 12 (96 Total)
Standard serum (ready-to-use)2 x 2 mL
Negative control serum (ready-to-use)2 mL
Anti-human-IgA-conjugate (ready-to-use)13 mL
Washing solution concentrate (sufficient for 1000ml)33.3 mL
Dilution buffer2 x 50 mL
Stopping solution15 mL
Substrate (ready-to-use)13 mL
Quality control certificate with standard curve and evaluation table1
Storage
Store at 2-8°C.
Associated products
Brucella abortus Antigen (BA116VS)
Brucella IgA Control Serum (BC116A)
Brucella IgG Control Serum (BC116G)
Brucella IgM Control Serum (BC116M)
Brucella IgA ELISA Kit (ESR116A)
Brucella IgG ELISA Kit (ESR116G)
Brucella IgM ELISA Kit (ESR116M)
target relevance
Organism
Brucella abortus
Structure and strains
Brucella abortus is a Gram-negative bacterium in the family Brucellaceae and is one of the causative agents of brucellosis. The rod-shaped pathogen is classified under the domain Bacteria. The prokaryotic B. abortus is non-spore-forming, non-motile and aerobic.
Disease
Brucella ssp. are non-motile bacteria which live as intracellular parasites in a wide spectrum of hosts, particularily farm animals. Infections of humans are mainly caused by Brucella melitensis (Malta fever), Brucella abortus (Morbus Bang), Brucella suis and Brucella canis. The is transmitted by contact with infected animals, their excretions and by contaminated food.

The disease starts with moderate fever. During the acute stage of the disease, the fever rises in the evenings. Additionally, hepatomegaly, splenomegaly or swollen lymph nodes are characteristic symptoms. In Brucella melitensis and Brucella suis infections, undulating fever within afebrile intervals may manifest. Spontaneous healing as well as transition into a chronic stage with a wide spectrum of symptoms is possible. Multiple organs or organ systems, bones or joints can be affected during the chronic stage. Histologically, characteristic granulomas may be observed in infected tissues. Bacterial endocarditis might be fatal if it remains untreated. During the late stage of brucellosis, neurologic and even psychiatric manifestations might occur.
Detection and diagnosis
Direct pathogen detection in culture systems can be performed with punctates from blood, bone marrow, synovia or urine. In this regard the special demands for nutrients have to be taken into account. More rapid results are obtained with serological methods such as agglutination tests. For differentiation between acute and chronic brucellosis, sensitive and specific ELISA test with separate detection of IgG, IgM and IgA antibodies are recommended.

Data

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
ESR116A protocol

Documents

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