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Doublecortin antibodies
Doublecortin (DCX) is a microtubule-associated protein that is critical for neuronal migration and neural development, particularly during brain formation. It is expressed in migrating neuroblasts and immature neurons, making it a valuable marker for neurogenesis. DCX plays a central role in stabilizing microtubules, which are essential for proper cell movement and positioning during brain development. Given its involvement in neurodevelopmental processes, doublecortin is widely studied in the context of brain disorders, such as epilepsy, lissencephaly, and other neuronal migration defects.In Western blotting (WB), doublecortin is commonly used to assess neuronal differentiation and migration during development or in response to injury. The doublecortin antibodies available on your website are also suitable for immunofluorescence (IF) and immunohistochemistry (IHC), allowing researchers to visualize the presence of immature neurons in brain tissue. These applications are particularly valuable in studies of neurodevelopment, brain plasticity, and neurogenesis, especially in regions like the hippocampus, where new neurons are generated throughout life.Due to its specific expression in migrating neurons and its crucial role in brain development, doublecortin is an important marker for investigating neurogenesis, neuronal migration disorders, and brain repair mechanisms. It is especially useful in research on developmental brain abnormalities, epilepsy, and neurodegenerative conditions where neurogenesis may be altered.
| target | type | reactivity | applications | ||||
|---|---|---|---|---|---|---|---|
| 9059 | Doublecortin | rabbit polyclonal | human mouse rat | WB ICC IHC | |||
WB with antibody [9059] : Detection of human Doublecortin by Western blot of immunoprecipitates.
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-N-BE(2) cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Doublecortin recombinant monoclonal antibody 9059 used for IP at 20 µl/mg lysate. Doublecortin was also immunoprecipitated by a second antibody against a different epitope of Doublecortin. For blotting immunoprecipitated Doublecortin, 9059 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.
ICC/IF with antibody [8001] : Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to doublecortin, 8001, dilution 1:1,000 in red, and costained with chicken pAb to microtubule associated protein 2 (MAP2), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. The doublecortin antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while the MAP2 antibody stains dendrites and perikarya of mature neurons. Doublecortin antibody is an excellent marker of early developing neuronal cells.
IHC with antibody [8001] : Chromogenic immunostaining of a 4% PFA fixed paraffin embedded rat testes section with mouse mAb to doublecortin, 8001, dilution 1:1,000, ddetected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. 8001 labels the Sertoli cells, the basement membrane and the late-stage spermatocytes. This antibody has not been tested in NBF fixed material.
