Human Yersinia IgA IgM Lateral flow dipstick kit 4673

$487.00

Summary

Mikrogen diagnostik lateral flow device (dipstick) for research use (RUO)
Human Yersinia IgA IgM Lateral flow dipstick kit 4673
Suitable for IgA/M detection
Ready-to-use
20 tests

SKU: 4673 Category: kits Tags: IgA/M, Lateral Flow Dipsticks, Yersinia

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	Human Yersinia IgA (IgM)
species reactivity	Yersinia enterocolitica, Yersinia pseudotuberculosis
applications	Lateral flow (dipstick)
assay type	Indirect & qualitative
available sizes	20 test kits

Human Yersinia IgA IgM Lateral flow dipstick kit 4673

Assay type
Sandwich assay, lateral flow (dipstick)

Research area
Infectious Disease

Sample type
Serum, plasma, whole blood

Components

10X Wash Buffer	100 mL
TMB Substrate	40 mL
Milk Powder	5 g
Instructions for Use	1 Each
Evaluation Form	1 Each
Test Strips	2 kits of [2 Vials x 10 Each]
Anti-Human IgM Conjugate	500 ?L
Positive Control	140 ?L
Negative Control	140 ?L

Storage
Store at 2-8°C.

Additional information
Mikrogen recomLine Yersinia Assays

For the Mikrogen recomLine Yersinia assays, the plasmid-encoded virulence proteins (Yersinia Outer Proteins) localized at the cell surface and adhesins for serological species differentiation between Y. enterocolitica and Y. pseudotuberculosis are produced recombinantly. These proteins are expressed only by human pathogenic Yersinia strains.

The recomLine Yersinia tests allow the detection of long-standing Yersinia infections and are thus ideally suited to identify Yersinia-induced immunopathological complications and chronic yersinioses. The detection of IgG and IgA antibodies can be an important aid in suspected Yersinia-induced arthritis.

Advantages

Detection of all human pathogenic Yersinia by means of Yersinia Outer Proteins (YOPs).
Differentiation between Y. enterocolitica and Y. pseudotuberculosis infection serologically possible by using new species-specific Yersinia antigens (PsaA, MyfA).
No cross-reactions to Brucella and other pathogens, and no interference by LPS.
Separate detection of IgG, IgA, and IgM antibodies - antibody class control on the test strip - helpful in case of suspected Yersinia-induced arthritis.
Partial and full automation, software-based evaluation (recomScan), and connection to the laboratory information system possible.
Highest sensitivity and specificity by using different, recombinant Yersinia antigens.

Bands

Antigen	Description
YOP M	Yersinia outer protein
V-AG	Yersinia virulence factor
PsaA	Adhesin (specific for Y. pseudotuberculosis)
YOP D	Yersinia outer protein
MyfA	Adhesin (specific for Y. enterocolitica)
YOP E	Yersinia outer protein

target relevance
Organism Yersinia enterocolitica
Structure and strains Yersinia enterocolitica is a Gram-negative, rod-shaped bacterium, belonging to the family Yersiniaceae. It is motile at temperatures of 22 29°C (72 84°F), but becomes nonmotile at normal human body temperature. Y. enterocolitica infection causes the disease yersiniosis, which is an animal-borne disease occurring in humans, as well as in a wide array of animals such as cattle, deer, pigs, and birds. Many of these animals recover from the disease and become carriers; these are potential sources of contagion despite showing no signs of disease. The bacterium infects the host by sticking to its cells using trimeric autotransporter adhesins.
Disease Yersinia are distributed globally in temperate and subtropical areas. The gram-negative bacteria express a range of plasmidencoded virulence factors, referred to as Yersinia Outer Proteins (YOPs). These molecules influence signal cascades within cells and inhibit the immune response of the host. The human pathogenic bacteria Y. enterocolitica and Y. pseudotuberculosis may cause inflammation of the intestine (enteritis) associated with stomach pain, diarrhea and vomiting. The disease symptoms are often difficult to distinguish from appendicitis (pseudoappendicitis). Possible postinfectious sequelae include reactive arthritis and erythema nodosum. These complications are frequently apparent in individuals carrying the HLA-B27 gene. Furthermore, cases of acute glomerulonephritis and myocarditis have been described. In patients with weakened immune systems, the disease may take a septic course.
Detection and diagnosis The use of sensitive and specific ELISA with quantitative determination of IgG, IgM and IgA antibodies is the method of choice for a clear differentiation between acute and chronic yersiniosis. The determination of the individual antibody activities is important for the differential diagnosis of post-infectious sequelae such as rheumatoid arthritis or erythema nodosum. Following the normal course of an acute Yersinia infection, IgG antibody activity often persists for several years while the IgA and IgM antibody activities decrease within a few months post infection. By contrast, in the case of postinfection complications, the IgA antibody activity remains elevated for years.

Data

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.