Monkeypox virus E8L Protein 7007

$283.00 – $3,200.00

Summary

Expression: Mammalian
Pure: Yes (HPLC)
Amino Acid Range: Met1-Thr275

SKU: 7007parent Categories: proteins, Recombinant proteins Tag: monkeypox virus

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	AIE40718.1
express system	Mammalian
product tag	C-FLAG
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Monkeypox is a zoonotic disease caused by monkeypox virus (MPXV), which is a member of orthopoxvirus genus. The MPXV E8L protein is the homologous protein of vaccinia virus D8L, D8L protein binds to chondroitin sulfate (CS) on cells, revealing a different type of virus-GAG interaction.The D8L protein has also been shown to elicit strong humoral and cellular immune responses in mice. This abundant 32-kDa IMV protein plays an important role in virus entry and adsorption due to its ability to bind to chondroitin sulfate on host cells. In vitro, antibodies directed against D8L have IMV-neutralizing activity, and soluble D8L protein inhibits the adsorption of wild-type vaccinia virus to cells. In vivo, D8L is immunogenic, and immunization of mice with a DNA vaccine encoding D8L provides partial protection from lethal vaccinia virus challenge. A study by Sakhatskyy has confirmed that D8L induces strong protective antibody responses in vivo and improves the efficacy of polyvalent poxvirus DNA vaccines composed of A27L and B5R or of A27L, B5R, L1R, and A33R
molecular weight	32.7 kDa
available size	1000 µg, 50 µg, 500 µg

Monkeypox E8L Protein 7007

protein

Tested applications WB,ELISA
Size and concentration 50, 500, 1000µg and 1 mg/mL
Storage buffer Supplied as a 0.2 µm filtered solution of PBS, pH7.4.
Purity > 95% as determined by SDS-PAGE

target relevance

Structure
The monkeypox virus is a large, double-stranded DNA virus within the Orthopoxvirus genus, known for its complex structure and multiple subunits that contribute to its infectivity and immune evasion. The virus is brick-shaped, with dimensions of approximately 200-250 nanometers, and is enveloped by a lipid bilayer that contains various glycoproteins essential for host cell entry. Within this outer envelope lies the core, or nucleoid, which houses the viral genome. This core is flanked by two lateral bodies that carry enzymes and proteins vital for early viral replication and manipulation of the host's cellular environment. The viral genome itself is extensive, encoding over 190 proteins that are crucial for the virus's life cycle, from entry and replication to immune evasion and virulence.

Key structural proteins encoded by the monkeypox virus genome include B6R, A35R, and M1R, among others. The B6R protein is an envelope glycoprotein involved in the formation of the extracellular enveloped virus (EEV), a form that enhances viral spread within the host by evading the immune system. The A35R protein plays a role in modulating the host immune response, particularly by interfering with the host's ability to mount an effective defense, thereby allowing the virus to establish a more persistent infection. The M1R protein, also known as the core protein, is crucial for maintaining the structural integrity of the viral core, ensuring that the viral DNA is protected and efficiently replicated within the host cell. Together, these and other viral proteins orchestrate a complex interplay of mechanisms that enable the monkeypox virus to infect host cells, replicate, and spread, making it a highly efficient pathogen.

Biotechnology
Diagnosis of monkeypox involves a combination of clinical assessment, laboratory testing, and advanced molecular techniques. Clinically, healthcare providers assess symptoms such as fever, swollen lymph nodes, and a distinctive rash that progresses through stages like macules, papules, vesicles, and pustules. Due to the similarity of these symptoms to other conditions like chickenpox, laboratory confirmation is crucial. Polymerase chain reaction (PCR) testing is the most reliable method, detecting viral DNA in samples from skin lesions, blood, or other bodily fluids. Additionally, serological methods, including antibody enzyme-linked immunosorbent assay (ELISA), are used to detect specific antibodies against the monkeypox virus. These tests help determine if an individual has been previously exposed to the virus or is in the later stages of infection. ELISA and other serological assays are valuable for both acute and retrospective diagnosis, providing insights into the immune response and potential immunity. In some cases, electron microscopy or virus isolation in cell culture may be utilized, particularly in research settings, to confirm the presence of the virus and study its characteristics.

Data

SDSPAGE Protein purity

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.