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SDS-PAGE – benchmark antibodies
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Blotting Out Uncertainties: Troubleshooting Common Western Blotting Issues

Western blotting is a powerful, tried-and trusted technique for studying proteins for more information about experimental design see our guide, but even the most seasoned researchers encounter challenges along the way. In this guide, we’ll explore common troubleshooting issues associated with Western blotting and share… Read More »Blotting Out Uncertainties: Troubleshooting Common Western Blotting Issues

milk powder used as blocking agent

Surface and membrane blocking

Non-specific protein blocking is a crucial step in various molecular biology and immunoassay techniques, including Western blotting and immunohistochemistry. It involves the pre-treatment of a biological sample or a membrane to prevent non-specific binding of antibodies or other detection molecules to unwanted proteins or surfaces.… Read More »Surface and membrane blocking

The Science of Transferring Proteins: From Acrylamide Gel onto Nitrocellulose

In the realm of molecular biology and protein research, the transfer of proteins from an acrylamide gel onto a nitrocellulose membrane is a pivotal step that opens up a world of possibilities for further analysis. This technique, known as Western blotting or immunoblotting, allows scientists… Read More »The Science of Transferring Proteins: From Acrylamide Gel onto Nitrocellulose