Western blotting is a powerful, tried-and trusted technique for studying proteins for more information about experimental design see our guide, but even the most seasoned researchers encounter challenges along the way. In this guide, we’ll explore common troubleshooting issues associated with Western blotting and share… Read More »Blotting Out Uncertainties: Troubleshooting Common Western Blotting Issues
Non-specific protein blocking is a crucial step in various molecular biology and immunoassay techniques, including Western blotting and immunohistochemistry. It involves the pre-treatment of a biological sample or a membrane to prevent non-specific binding of antibodies or other detection molecules to unwanted proteins or surfaces.… Read More »Surface and membrane blocking
In the realm of molecular biology and protein research, the transfer of proteins from an acrylamide gel onto a nitrocellulose membrane is a pivotal step that opens up a world of possibilities for further analysis. This technique, known as Western blotting or immunoblotting, allows scientists… Read More »The Science of Transferring Proteins: From Acrylamide Gel onto Nitrocellulose
When it comes to creating polyacrylamide gels for electrophoresis, there’s a crucial ingredient that takes center stage – acrylamide. This simple organic compound is the cornerstone of PAGE (Polyacrylamide Gel Electrophoresis) gels, serving as the foundational building block. The magic of acrylamide lies in its… Read More »The basics of acrylamide
In the world of molecular biology and biochemistry, one technique reigns supreme when it comes to separating proteins by size: SDS-PAGE, or Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis. For years this method has provided a reliable means to analyze complex protein mixtures and determine their… Read More »How SDS-PAGE works