Introduction: Western blotting is a powerful, tried-and trusted technique for studying proteins for more information about experimental design see our guide. In this blog post, we will delve into the science behind nitrocellulose’s role in protein research, explore how it works in this context, uncover its… Read More »Nitrocellulose in Protein Research: An Explosive Blend of Science and History
Non-specific protein blocking is a crucial step in various molecular biology and immunoassay techniques, including Western blotting and immunohistochemistry. It involves the pre-treatment of a biological sample or a membrane to prevent non-specific binding of antibodies or other detection molecules to unwanted proteins or surfaces.… Read More »Surface and membrane blocking
In the realm of molecular biology and protein research, the transfer of proteins from an acrylamide gel onto a nitrocellulose membrane is a pivotal step that opens up a world of possibilities for further analysis. This technique, known as Western blotting or immunoblotting, allows scientists… Read More »The Science of Transferring Proteins: From Acrylamide Gel onto Nitrocellulose