| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Human IgG H&L Chain |
| available sizes | 0.5 mg |
goat anti-human IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1707
$208.00
Antibody summary
- Goat polyclonal to Human IgG, H&L Chain Specific, Peroxidase
- crossabsorbed
- Suitable for: WB,IHC,ICC,ELISA
- Isotype: Whole IgG
- 0.5 mg
goat anti-human IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1707
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunohistochemistry: use at a dilution of 1:50-1:500 Immunocytochemistry: use at a dilution of 1:50-1:500. Immunofluorescence: use at a dilution of 1:50-1:500. These are recommended dilutions. End users should determine optimal dilutions for their applications. Prep |
| Immunogen Human IgG |
| Size and concentration 0.5mg and 1 mg/mL |
| Form liquid |
| Storage Instructions Store at 2-8°C. Shelf life is one (1) year from date of receipt. |
| Storage buffer Phosphate Buffered Saline (PBS) containing 0.05% Proclin 400 |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries NA |
| Isotype control NA |
| target relevance |
|---|
| human IgG |
| Protein names Immunoglobulin G |
| Alternative names IgG, Gamma immunoglobulin, Gamma globulin |
| Function IgG antibodies bind antigens with high specificity and mediate immune protection through pathogen neutralization, opsonization, complement activation, antibody-dependent cellular cytotoxicity (ADCC), and immune complex formation. |
| Subcellular location Secreted; found predominantly in blood plasma, lymph, extracellular fluids, and tissues. |
| Structure IgG is a heterotetrameric glycoprotein composed of two identical gamma heavy chains and two identical light chains linked by disulfide bonds. The molecule contains two antigen-binding Fab regions and one Fc region. |
| Description Immunoglobulin G (IgG) is the most abundant antibody isotype in human serum and extracellular fluids. It plays a central role in adaptive immunity by recognizing antigens, neutralizing pathogens, activating effector mechanisms, and providing long-term immune protection. |
| Related entities relationship: has_subclass; entity: IgG1 relationship: has_subclass; entity: IgG2 relationship: has_subclass; entity: IgG3 relationship: has_subclass; entity: IgG4 relationship: related_to; entity: IgA relationship: related_to; entity: IgM relationship: related_to; entity: IgE relationship: related_to; entity: IgD |
| Keywords Antibody, Immunoglobulin, Adaptive immunity, Humoral immunity, Complement activation |
| Structure IgG is composed of two gamma heavy chains and two light chains arranged into a Y-shaped antibody molecule containing Fab and Fc regions. |
| Function IgG mediates antigen recognition, pathogen neutralization, opsonization, complement activation, and Fc receptor-dependent immune responses. |
| Subclasses Human IgG is divided into four subclasses: IgG1, IgG2, IgG3, and IgG4, each possessing distinct effector functions and antigen preferences. |
| Distribution IgG is the most abundant immunoglobulin in serum and extracellular fluids and is the dominant antibody during secondary immune responses. |
| Research relevance IgG is widely studied in immunology, infectious disease, autoimmunity, oncology, vaccine development, and therapeutic antibody research. |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What species and immunoglobulin chains does this goat anti-human IgG antibody specifically recognize?
This antibody specifically recognizes human IgG heavy and light (H&L) chains.
Which applications has this peroxidase conjugated antibody been validated for, and what are the recommended dilution ranges?
The antibody is validated for Western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC/IF), and ELISA. Recommended dilutions for IHC, ICC, and immunofluorescence are between 1:50 and 1:500, though users should optimize dilutions for their specific applications.
What are the storage conditions and shelf life for maintaining the stability of this peroxidase conjugated antibody?
The antibody should be stored refrigerated at 2-8°C, and it has a shelf life of one year from the date of receipt. It is supplied in phosphate buffered saline (PBS) containing 0.05% Proclin 400 as a preservative.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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