| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgY |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Chicken IgY H&L Chain |
| available sizes | 0.5 mg |
goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1718
$208.00
Antibody summary
- Goat polyclonal to Chicken IgG, H&L Chain Specific, Biotin
- crossabsorbed
- Suitable for: WB,IHC,ICC,ELISA
- Isotype: Whole IgG
- 0.5 mg
goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1718
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunohistochemistry: use at a dilution of 1:50-1:500 Immunocytochemistry: use at a dilution of 1:50-1:500. Immunofluorescence: use at a dilution of 1:50-1:500. These are recommended dilutions. End users should determine optimal dilutions for their applications. Prep |
| Immunogen Chicken IgY |
| Size and concentration 0.5mg and 1 mg/mL |
| Form liquid |
| Storage Instructions Store at 2-8°C. Shelf life is one (1) year from date of receipt. |
| Storage buffer Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries NA |
| Isotype control NA |
| target relevance |
|---|
| chicken IgY |
| Protein names Chicken immunoglobulin Y |
| Alternative names IgY, Chicken IgY, Gallus gallus IgY, Avian IgY |
| Function Chicken IgY mediates humoral immune responses by specifically recognizing antigens and promoting pathogen neutralization, immune clearance, and protection against infection. |
| Subcellular location Secreted; present in blood plasma, extracellular fluids, and highly concentrated in egg yolk. |
| Structure IgY is composed of two heavy chains and two light chains linked by disulfide bonds. Although functionally analogous to mammalian IgG, the IgY heavy chain contains four constant domains and lacks a classic mammalian hinge region. |
| Description Immunoglobulin Y (IgY) is the major serum antibody of birds, reptiles, and amphibians. In chickens, IgY serves a role analogous to mammalian IgG and is the predominant antibody transferred from the hen to the egg yolk, providing passive immunity to developing offspring. |
| Related entities relationship: evolutionary_related_to; entity: Immunoglobulin G relationship: found_in; entity: Egg yolk |
| Keywords Antibody, Immunoglobulin, IgY, Humoral immunity, Passive immunity |
| Structure Chicken IgY is a tetrameric antibody composed of two heavy chains and two light chains. Unlike mammalian IgG, IgY contains four constant domains and lacks a classical hinge region. |
| Function IgY serves as the principal antibody of birds and mediates antigen recognition, pathogen neutralization, and humoral immune protection. |
| Distribution IgY is found in chicken serum and is actively transported into egg yolk to provide maternal immunity to developing chicks. |
| Advantages IgY antibodies typically do not bind mammalian Fc receptors, rheumatoid factors, or complement components, reducing background interference in many immunoassays. |
| Research relevance Chicken IgY is widely used in ELISA, western blotting, immunohistochemistry, lateral flow assays, passive immunization studies, and antibody production because large quantities can be obtained non-invasively from egg yolks. |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What species and antibody target does this goat anti-chicken IgY antibody specifically recognize?
This antibody specifically recognizes the heavy and light chains of chicken Immunoglobulin Y (IgY).
Which applications has this biotin-conjugated goat anti-chicken IgY antibody been validated for?
It has been tested and is suitable for Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry/immunofluorescence (ICC/IF), and ELISA applications.
What are the recommended dilution ranges for using this antibody in immunohistochemistry and immunofluorescence?
For both immunohistochemistry and immunofluorescence, the recommended dilution range is between 1:50 and 1:500. However, end users should optimize dilutions based on their specific experimental conditions.
How should this goat anti-chicken IgY antibody be stored to maintain stability and shelf life?
The antibody should be stored refrigerated at 2-8°C, where it remains stable for up to one year from the date of receipt.
What is the composition of the storage buffer for this polyclonal antibody, and does it contain any preservatives?
The antibody is supplied in phosphate buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.09% sodium azide as a preservative.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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