Sample type Serum, Plasma, Cell Culture Supernatant, Other liquid samples
Components
Break apart microtiter test strips each coated single wells
8 x 12 (96 Total)
Lyophilized Standard
2 x vial
Biotin-labeled Antibody(Concentrated, 100X)
120 uL
HRP-Streptavidin Conjugate(Concentrated, 100X)
120 uL
Washing solution concentrate (25X)
30 mL
Sample Dilution buffer
20 mL
Antibody Dilution buffer
10 mL
Streptavidin Dilution buffer
10 mL
Stopping solution
10 mL
TMB Substrate (ready-to-use)
10 mL
Plate seals
3
Storage Store at 2-8°C.
target relevance
anti-Vanucizumab antibody Anti-drug antibodies (ADAs) generated in subjects following administration of Vanucizumab.
Vanucizumab Vanucizumab biologic drug binds Homo sapiens VEGFA,Homo sapiens VPS51
Homo sapiens VEGFA Vascular endothelial growth factor A, long form
Protein names Vascular endothelial growth factor A, long form
Alternative names Vascular permeability factor
Gene names VEGFA
Protein family Belongs to the PDGF/VEGF growth factor family
Function Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed:35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity)
Subcellular location Secreted
Structure Homodimer; disulfide-linked (By similarity). Also found as heterodimer with PGF (By similarity). Interacts with NRP1 (PubMed:26503042). Interacts with isoform 2 of BSG (PubMed:25825981). Interacts with CD82; this interaction inhibits VEGFA-mediated signaling pathway (PubMed:34530889)
Post-translational modification Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form
Involvement in disease Microvascular complications of diabetes 1 Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
Keywords 3D-structure, Alternative initiation, Alternative promoter usage, Alternative splicing, Angiogenesis, Cytoplasm, Developmental protein, Differentiation, Direct protein sequencing, Disulfide bond, Endoplasmic reticulum, Extracellular matrix, Glycoprotein, Golgi apparatus, Growth factor, Heparin-binding, Mitogen, Nucleus, Proteomics identification, Reference proteome, Secreted
Homo sapiens VPS51 Vacuolar protein sorting-associated protein 51 homolog
Protein names Vacuolar protein sorting-associated protein 51 homolog
Alternative names Another new gene 2 protein, Protein fat-free homolog
Gene names VPS51
Protein family Belongs to the VPS51 family
Function Acts as a component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. VPS51 participates in retrograde transport of acid hydrolase receptors, likely by promoting tethering and SNARE-dependent fusion of endosome-derived carriers to the TGN (PubMed:20685960). Acts as a component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane (PubMed:25799061)
Structure Component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54 (PubMed:20685960, PubMed:27440922, PubMed:30624672). Component of the endosome-associated retrograde protein (EARP) complex, composed of VPS51, VPS52, VPS53 and VPS50/Syndetin (PubMed:25799061, PubMed:27440922, PubMed:30624672). EIPR1 interacts with both EARP and GARP complexes and mediates the recruitment of the GARP complex to the trans-Golgi network (PubMed:27440922). Interacts with STX6 (via N-terminus) (PubMed:20685960). Interacts with VPS50 and VPS54 in an EIPR1-independent manner (PubMed:31721635)
Involvement in disease Pontocerebellar hypoplasia 13 A form of pontocerebellar hypoplasia, a disorder characterized by structural defects of the pons and cerebellum, evident upon brain imaging. PCH13 is an autosomal recessive form characterized by delayed psychomotor development, absent speech, severe intellectual disability and postnatal microcephaly, with brain malformations consisting of cerebellar atrophy and hypoplastic corpus callosum. Additional features, including seizures and visual impairment, are variable.
Keywords 3D-structure, Acetylation, Alternative splicing, Coiled coil, Disease variant, Endosome, Golgi apparatus, Intellectual disability, Lipid transport, Phosphoprotein, Protein transport, Proteomics identification, Reference proteome, Transport
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Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
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