Anti-Numab patent anti-HSA antibody ELISA Kit 60613

$570.00

Summary

ELISA kit for research use (RUO)
Reactivity: Anti-Numab patent anti-HSA Ab
Suitable for: ELISA
Time:3 hours
Sample types:Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

SKU: 60613 Category: Bridging ELISA Tags: ADA, Anti-Numab patent anti-HSA Antibodies, biologics, Numab patent HSA

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	Anti-Numab patent anti-HSA Ab
species reactivity	Universal
applications	Double antigen, Sandwich ELISA
assay type	direct & quantitative
available size	96 tests
sensitivity	0.938ng/ml
range	1.563-100ng/ml

Anti-Numab patent anti-HSA antibody ELISA Kit

Assay type
Bridging ELISA

Research area
biologic

Sample type
Serum, Plasma, Cell Culture Supernatant, Other liquid samples

Components

Break apart microtiter test strips each coated single wells	8 x 12 (96 Total)
Lyophilized Standard	2 x vial
Biotin-labeled Antibody(Concentrated, 100X)	120 uL
HRP-Streptavidin Conjugate(Concentrated, 100X)	120 uL
Washing solution concentrate (25X)	30 mL
Sample Dilution buffer	20 mL
Antibody Dilution buffer	10 mL
Streptavidin Dilution buffer	10 mL
Stopping solution	10 mL
TMB Substrate (ready-to-use)	10 mL
Plate seals	3

Storage
Store at 2-8°C.

target relevance

anti-Numab patent anti-HSA antibody
Anti-drug antibodies (ADAs) generated in subjects following administration of Numab patent anti-HSA.

Numab patent anti-HSA
Numab patent anti-HSA biologic drug binds Homo sapiens ALB Albumin

Homo sapiens ALB
Albumin

Protein names
Albumin

Gene names
ALB

Protein family
Belongs to the ALB/AFP/VDB family

Function
Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017)

Subcellular location
Secreted

Structure
Interacts with FCGRT; this interaction regulates ALB homeostasis (PubMed:28330995). Interacts with TASOR (By similarity). In plasma, occurs in a covalently-linked complex with chromophore-bound alpha-1-microglobulin with molar ratio 1:2 and 1:1; this interaction does not prevent fatty acid binding to ALB

Post-translational modification
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606
Glycated in diabetic patients
Phosphorylated by FAM20C in the extracellular medium
Acetylated on Lys-223 by acetylsalicylic acid

Involvement in disease
Hyperthyroxinemia, familial dysalbuminemic
A disorder characterized by abnormally elevated levels of total serum thyroxine (T4) in euthyroid patients. It is due to abnormal serum albumin that binds T4 with enhanced affinity.

Analbuminemia
A rare autosomal recessive disorder manifested by the presence of a very low amount of circulating serum albumin. Affected individuals manifest mild edema, hypotension, fatigue, and, occasionally, lower body lipodystrophy (mainly in adult females). The most common biochemical finding is hyperlipidemia, with a significant increase in the total and LDL cholesterol concentrations, but normal concentrations of HDL cholesterol and triglycerides.

Keywords
3D-structure, Alternative splicing, Calcium, Cleavage on pair of basic residues, Copper, Direct protein sequencing, Disease variant, Disulfide bond, Glycation, Glycoprotein, Lipid-binding, Metal-binding, Methylation, Phosphoprotein, Proteomics identification, Reference proteome, Repeat, Secreted, Signal, Zinc

Sequence
MKWVTFISLLFLFSSAYSRGVFRRDAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPF EDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEP ERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLF FAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAV ARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLK ECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYAR RHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFE QLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVV LNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTL SEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLV AASQAALGL

UniProt accession: P02768

Data

Standard Curve

X axis: log | Y axis: linear

Standard	STD [ng/mL]	OD1	OD2	Average OD	Corrected OD
Blank #8	0	0.09	0.09	0.09	0
Standard #7	1.56	0.14	0.14	0.14	0.05
Standard #6	3.13	0.33	0.34	0.34	0.25
Standard #5	6.25	0.43	0.44	0.43	0.34
Standard #4	12.5	0.77	0.79	0.78	0.69
Standard #3	25	1.29	1.33	1.31	1.22
Standard #2	50	1.74	1.79	1.77	1.68
Standard #1	100	2.45	2.52	2.48	2.39

Precision

Sample	n	Mean	Standard Deviation	CV (%)
Intra Sample 1	20	3.04	0.14	4.69
Intra Sample 2	20	13.54	0.77	5.72
Intra Sample 3	20	49.52	2.45	4.95
Inter Sample 1	20	3.24	0.15	4.69
Inter Sample 2	20	13.96	0.73	5.23
Inter Sample 3	20	50.83	2.88	5.67

Recovery

Matrix	Recovery Range (%)	Average (%)
Serum(n=5)	85-99	92
EDTA Plasma(n=5)	85-104	97
HeparinPlasma(n=5)	86-103	94

Stability

37°C 1 Month (%)	2–8°C 6 Months (%)	2–8°C 12 Months (%)
80	95-100	85-98

Linearity

Sample	1:2 Dilution (%)	1:4 Dilution (%)	1:8 Dilution (%)
Serum(n=5)	85-100%	83-98%	82-98%
EDTAPlasma(n=5)	87-102%	85-101%	82-99%
Heparin Plasma(n=5)	86-102%	86-96%	82-96%

FAQ & Publications

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

Relevant to this product
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Documents

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Anti-Numab patent anti-HSA antibody ELISA Kit 60613

Data

Standard Curve

Precision

Recovery

Stability

Linearity

FAQ & Publications

Protocols

Documents

Reviews

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