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Myc tag antibodies
The Myc tag is a popular epitope tag derived from the c-Myc protein, consisting of a short amino acid sequence (EQKLISEEDL) that is widely used in molecular biology for the detection and purification of recombinant proteins. By attaching the Myc tag to a protein of interest, researchers can easily identify, purify, and study the protein using anti-Myc antibodies. The tag is particularly useful in Western blotting (WB), immunoprecipitation, and immunofluorescence (IF), providing a versatile tool for protein research.In Western blotting, Myc-tagged proteins are readily detected using specific anti-Myc antibodies. The Myc tag antibodies available on your website are also suitable for use in immunofluorescence (IF) and immunohistochemistry (IHC), enabling researchers to localize Myc-tagged proteins within cells or tissues. These applications are essential for protein expression studies, protein-protein interaction assays, and other functional analyses.The Myc tag is named after the c-Myc protein, a transcription factor that plays a critical role in cell growth, proliferation, and apoptosis. c-Myc regulates the expression of numerous genes involved in cell cycle progression, metabolism, and ribosome biogenesis. Given its involvement in promoting cell division, c-Myc is often dysregulated in various cancers, making it a target of interest in cancer research. By tagging proteins with the Myc sequence, researchers can leverage the specificity and flexibility of this epitope tag while also exploring broader cellular processes related to c-Myc, including oncogenesis and gene regulation. The Myc tag thus serves as both a practical tool and a connection to important biological pathways.
ICC/IF with antibody [8059] : Immunofluorescence cell staining rabbit anti-myc tag polyclonal antibody 8059
Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed, permeabilized with 0.1% triton X-100 in CHO cells transfected with construct labeling with MYC antibody 8059 at 1:1000 dilution (1µg), followed by donkey-anti rabbit IgG (H&L), FITC conjugated antibody at 1:1000 dilution (1µg/mL) (green). Nucleus was counterstained with DAPI (blue).
WB with antibody [5460] : 1:1,000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with Myc-tagged protein vector; untransfected (1) and transfected (2).
ICC/IF with antibody [1013] : Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed, permeabilized with 0.1% triton X-100 in CHO cells transfected with GFP-MYC construct (CHO-GFP-MYC) and parallel CHO cells (bottom). Labeling MYC with goat anti-myc tag polyclonal antibody 10131 (1.5µg/mL), followed by donkey anti-goat IgG (H&L),Alexa 594 antibody at dilution (3µg/mL) (red). Nucleus was counterstained with DAPI (blue).