When it comes to creating polyacrylamide gels for electrophoresis, there’s a crucial ingredient that takes center stage – acrylamide. This simple organic compound is the cornerstone of PAGE (Polyacrylamide Gel Electrophoresis) gels, serving as the foundational building block. The magic of acrylamide lies in its… Read More »The basics of acrylamide
In the world of molecular biology and biochemistry, one technique reigns supreme when it comes to separating proteins by size: SDS-PAGE, or Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis. For years this method has provided a reliable means to analyze complex protein mixtures and determine their… Read More »How SDS-PAGE works
The Importance of Loading Control Antibodies When conducting experiments, it is crucial to have accurate and reliable results. One way to ensure this is by using loading control antibodies. These antibodies are essential tools in Western blotting and other protein analysis techniques. What exactly do… Read More »The Use of Control Antibodies in Research
Loading control antibodies play a crucial role in Western blot analysis, where they serve as internal references to normalize protein expression levels. By targeting housekeeping proteins that are consistently expressed across different samples, loading control antibodies allow for accurate comparisons of protein levels between experimental… Read More »The Importance of Loading Control Antibodies