Western blotting is a powerful, tried-and trusted technique for studying proteins for more information about experimental design see our guide, but even the most seasoned researchers encounter challenges along the way. In this guide, we’ll explore common troubleshooting issues associated with Western blotting and share… Read More »Blotting Out Uncertainties: Troubleshooting Common Western Blotting Issues
Immunohistochemistry (IHC) is a powerful technique that allows scientists and researchers to visualize and analyze the distribution of specific proteins or antigens within tissue samples. One of the key steps in the IHC process is cell staining, where various staining methods are employed to enhance… Read More »Exploring the Spectrum of Cell Staining Techniques in Immunohistochemistry
When it comes to our cells’ resilience and ability to combat stress, there’s a group of molecular superheroes at play known as heat shock proteins (HSPs). These tiny chaperones might not wear capes, but they perform remarkable tasks within our cells. In this blog post,… Read More »Guardians of the Cell: Impressions of Hsp60
In the realm of molecular biology and biochemistry, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) often plays a silent yet indispensable role. It is commonly known for its use as a loading control in Western blots and other assays, but its significance goes far beyond that. This unassuming enzyme… Read More »Unveiling the Versatility of GAPDH: Beyond a Loading Control
Vimentin, often described as the “intermediate filament protein,” is a fascinating component of our cellular architecture that plays a pivotal role in maintaining cell structure and integrity. In this blog post, we will delve into the intricacies of vimentin’s structure and its indispensable functions within… Read More »Unraveling the Fibrous Beauty of Vimentin: A Closer Look at its Structure
When it comes to cellular function and structure, the dynamic duo of alpha and beta actin proteins take center stage. These closely related proteins, part of the actin family, play pivotal roles in ensuring that cells maintain their integrity and functionality. In this blog post,… Read More »Exploring the Dynamic Duo: Alpha and Beta Actin
Introduction Tubulin is a critical protein in the cell’s structural framework, known as the cytoskeleton. It plays a pivotal role in maintaining cell shape, facilitating intracellular transport, and participating in processes like cell division. Tubulin exists in various isoforms, each with unique functions and properties,… Read More »Tubulin Isoforms: Orchestrating Cellular Dynamics Through Microtubule Organization
Introduction Within the microscopic world of cells lies a complex framework known as the cytoskeleton, like the human skeleton it provides structural support but also orchestrates various cellular activities. At the heart of this cytoskeletal network are actin filaments, slender threads of protein that play… Read More »The Intricate Dance of Actin Filaments: Building Blocks of Cell Structure and Function
Decoding its 3D Structure, Fluorescence, and Color-Changing Mutants Green Fluorescent Protein (GFP) is a captivating molecule that has revolutionized the way we study cells and organisms. Its 3D structure, fluorescence mechanism, and remarkable mutants that change its color are a testament to the fascinating world… Read More »The magic of GFP – green fluorescent protein
In the world of molecular biology and biomedical research, antibody-horseradish peroxidase (HRP) conjugates have emerged as indispensable tools for various applications, including immunoassays, immunohistochemistry, and Western blotting. To appreciate their significance fully, one must delve into the chemistry of the peroxidase reaction that powers these conjugates. In this blog post, we’ll explore HRP chemistry, shedding light on the catalytic process that makes antibody-HRP conjugates invaluable in the laboratory.