Western blotting is a powerful, tried-and trusted technique for studying proteins for more information about experimental design see our guide, but even the most seasoned researchers encounter challenges along the way. In this guide, we’ll explore common troubleshooting issues associated with Western blotting and share… Read More »Blotting Out Uncertainties: Troubleshooting Common Western Blotting Issues
Calreticulin (CRT) is a multifunctional protein with a highly conserved structure across different species. It primarily resides in the endoplasmic reticulum (ER), but it can also be found in other cellular locations. CRT’s structure and functions can be broken down as follows: Structure: CRT is… Read More »Revealing the Multifaceted World of Calreticulin: A Protein with Many Roles
Decoding its 3D Structure, Fluorescence, and Color-Changing Mutants Green Fluorescent Protein (GFP) is a captivating molecule that has revolutionized the way we study cells and organisms. Its 3D structure, fluorescence mechanism, and remarkable mutants that change its color are a testament to the fascinating world… Read More »The magic of GFP – green fluorescent protein
In the world of molecular biology and biomedical research, antibody-horseradish peroxidase (HRP) conjugates have emerged as indispensable tools for various applications, including immunoassays, immunohistochemistry, and Western blotting. To appreciate their significance fully, one must delve into the chemistry of the peroxidase reaction that powers these conjugates. In this blog post, we’ll explore HRP chemistry, shedding light on the catalytic process that makes antibody-HRP conjugates invaluable in the laboratory.
In the realm of life sciences and medical research, the development of innovative tools and techniques has been instrumental in advancing our understanding of biology and improving diagnostics and therapies. Antibody conjugates, specifically those linked to enzymes and fluorophores, represent a groundbreaking fusion of biology… Read More »Highlighting the Power of Antibody Conjugates: Enzymes and Fluorophores
Non-specific protein blocking is a crucial step in various molecular biology and immunoassay techniques, including Western blotting and immunohistochemistry. It involves the pre-treatment of a biological sample or a membrane to prevent non-specific binding of antibodies or other detection molecules to unwanted proteins or surfaces.… Read More »Surface and membrane blocking
The Importance of Loading Control Antibodies When conducting experiments, it is crucial to have accurate and reliable results. One way to ensure this is by using loading control antibodies. These antibodies are essential tools in Western blotting and other protein analysis techniques. What exactly do… Read More »The Use of Control Antibodies in Research