In the realm of life sciences and medical research, the development of innovative tools and techniques has been instrumental in advancing our understanding of biology and improving diagnostics and therapies. Antibody conjugates, specifically those linked to enzymes and fluorophores, represent a groundbreaking fusion of biology… Read More »Highlighting the Power of Antibody Conjugates: Enzymes and Fluorophores
Introduction: Our immune system is a remarkable fortress, tirelessly guarding our bodies against an army of invading pathogens. It consists of two fundamental lines of defense: the innate and acquired immune systems, working in harmony to keep us healthy. In this blog post, we’ll delve… Read More »Navigating the Immune System: Innate and Acquired Defenses
Immunoglobulins, often referred to as antibodies, are essential components of our immune system, responsible for defending our bodies against a wide range of pathogens and foreign invaders. These remarkable molecules come in various classes, each with a unique structure and function tailored to combat different types of threats. The five major classes of immunoglobulins are IgG, IgM, IgA, IgD, and IgE.
IgG antibodies, a subclass of immunoglobulins, are essential tools in the realm of scientific research, thanks to their remarkable structure and versatility. These antibodies have a well-defined domain organization that allows them to perform various crucial functions. At the core of their structure, IgG antibodies feature two distinct domains: the variable (V) and constant (C) domains.
Secondary antibodies are crucial in a multitude of applications, particularly in immunodetection techniques like Western blotting, immunohistochemistry and immunofluorescence. Their primary function is to amplify the signal generated by the binding of primary antibodies to specific target molecules. This amplification is pivotal for visualizing and quantifying proteins or other biomolecules within a sample. One essential aspect of secondary antibodies’ reliability is their manufacturing process, which often involves affinity purification.
Non-specific protein blocking is a crucial step in various molecular biology and immunoassay techniques, including Western blotting and immunohistochemistry. It involves the pre-treatment of a biological sample or a membrane to prevent non-specific binding of antibodies or other detection molecules to unwanted proteins or surfaces.… Read More »Surface and membrane blocking
In the realm of molecular biology and protein research, the transfer of proteins from an acrylamide gel onto a nitrocellulose membrane is a pivotal step that opens up a world of possibilities for further analysis. This technique, known as Western blotting or immunoblotting, allows scientists… Read More »The Science of Transferring Proteins: From Acrylamide Gel onto Nitrocellulose
When it comes to creating polyacrylamide gels for electrophoresis, there’s a crucial ingredient that takes center stage – acrylamide. This simple organic compound is the cornerstone of PAGE (Polyacrylamide Gel Electrophoresis) gels, serving as the foundational building block. The magic of acrylamide lies in its… Read More »The basics of acrylamide
In the world of molecular biology and biochemistry, one technique reigns supreme when it comes to separating proteins by size: SDS-PAGE, or Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis. For years this method has provided a reliable means to analyze complex protein mixtures and determine their… Read More »How SDS-PAGE works