Secondary antibodies are crucial in a multitude of applications, particularly in immunodetection techniques like Western blotting, immunohistochemistry and immunofluorescence. Their primary function is to amplify the signal generated by the binding of primary antibodies to specific target molecules. This amplification is pivotal for visualizing and quantifying proteins or other biomolecules within a sample. One essential aspect of secondary antibodies’ reliability is their manufacturing process, which often involves affinity purification.