| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Mouse IgG H&L Chain |
| available sizes | 1 mg |
goat anti-mouse IgG, H&L chain specific, alk phos conjugated polyclonal antibody 3184
$231.00
Antibody summary
- Goat polyclonal to Mouse IgG H&L Chain, Alk Phos
- Suitable for: WB,IHC,ICC,ELISA
- Isotype: Whole IgG
- 1 mg
goat anti-mouse IgG, H&L chain specific, alk phos conjugated polyclonal antibody 3184
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions ELISA: 1:1,000-1:10,000 Western Blot: 1:1,000-1:10,000 Immunohistochemistry: 1:200-1:1,000 Immunocytochemistry: 1:200-1:1,000. Prepare working dilutions immediately before use. These are recommended dilutions. User should determine optimal dilutions for their |
| Immunogen Mouse IgG |
| Size and concentration 1mg and 1 mg/mL |
| Form liquid |
| Storage Instructions Store at 2-8°C. Shelf life is one (1) year from date of receipt. |
| Storage buffer 50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705 |
| Isotype control Goat polyclonal - Isotype Control |
| target relevance |
|---|
| mouse IgG |
| Protein names Mouse immunoglobulin G |
| Alternative names Mouse IgG, Mus musculus IgG, Mouse gamma immunoglobulin |
| Function Mouse IgG antibodies specifically recognize antigens and mediate immune protection through neutralization, opsonization, complement activation, antibody-dependent cellular cytotoxicity, and immune complex formation. |
| Subcellular location Secreted; present in blood plasma, lymph, extracellular fluids, and tissues. |
| Structure Mouse IgG is composed of two identical gamma heavy chains and two identical light chains linked by disulfide bonds, forming a Y-shaped immunoglobulin molecule containing Fab and Fc regions. |
| Description Mouse immunoglobulin G (IgG) is the predominant antibody isotype found in mouse serum and extracellular fluids. It plays a central role in humoral immunity through antigen recognition, pathogen neutralization, complement activation, and Fc receptor-mediated effector functions. |
| Related entities relationship: has_subclass; entity: Mouse IgG1 relationship: has_subclass; entity: Mouse IgG2a relationship: has_subclass; entity: Mouse IgG2b relationship: has_subclass; entity: Mouse IgG3 relationship: part_of; entity: Immunoglobulin G |
| Keywords Antibody, Immunoglobulin, Humoral immunity, Adaptive immunity |
| Structure Mouse IgG is a tetrameric antibody consisting of two gamma heavy chains and two light chains linked by disulfide bonds. |
| Function Mouse IgG mediates antigen recognition, pathogen neutralization, opsonization, complement activation, and Fc receptor-dependent immune responses. |
| Subclasses Mouse IgG is divided into four subclasses: IgG1, IgG2a, IgG2b, and IgG3, each exhibiting distinct biological and effector properties. |
| Distribution Mouse IgG is the major immunoglobulin isotype found in mouse serum and extracellular fluids. |
| Research relevance Mouse IgG is extensively used in monoclonal antibody production, immunology research, ELISA, western blotting, immunohistochemistry, flow cytometry, and therapeutic antibody development. Anti-mouse IgG secondary antibodies are among the most commonly used detection reagents in biomedical research. |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What applications is the goat anti-mouse IgG H&L chain specific alkaline phosphatase conjugated polyclonal antibody suitable for?
This antibody is suitable for Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry/Immunofluorescence (ICC/IF), and ELISA applications.
How should this antibody be stored to maintain its stability?
The antibody should be stored at 2-8°C and has a shelf life of one year from the date of receipt.
What is the recommended dilution range for using this antibody in ELISA assays?
For ELISA applications, the recommended dilution range is between 1:1,000 and 1:10,000, and working dilutions should be prepared immediately before use.
What species does this secondary antibody specifically recognize?
This antibody specifically recognizes mouse IgG heavy and light chains.
What is the host species and clonality of this anti-mouse IgG antibody?
The antibody is a goat polyclonal antibody with whole IgG isotype.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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