Campylobacter jejuni IgG ELISA Kit MD-6204

Campylobacter jejuni IgG ELISA Kit MD-6204

kit
Assay type Indirect ELISA
Research area Infectious Disease
Sample type Serum, plasma, whole blood
Components Wash buffer (ten-times concentration) Contains phosphate buffer, NaCl, detergent, preservative: MIT (0.01%) and Oxypyrion (0.1%) 100 mL Dilution buffer (ready-to-use) Contains protein, detergent and blue dye. Preservative: MIT (0.01%) and Oxypyrion (0.1%) 125 mL Chromogenic substrate tetramethylbenzidine (TMB, ready-to-use) 12 mL Stop solution 24.9% phosphoric acid (H?PO?) (ready-to-use) 12 mL Instructions for use 1 Evaluation form 1 Covering film 2 pieces recomWell HEV IgG also contains: 12 x 8 wells microplate (section marked in red), coated with recombinant HEV antigens in a vacuum-pressure-sealed bag 1 Positive control (violet cap) Contains MIT (0.1%) and Oxypyrion (0.1%) 450 µ L Cutoff control (yellow cap) Contains MIT (0.1%) and Oxypyrion (0.1%) 450 µ L Negative control (white cap) Contains MIT (0.1%) and Oxypyrion (0.1%) 450 µ L Anti-human IgG conjugate (101x concentrated, red cap) Contains NaN? (<0.1%), MIT (<0.01%) and chlorazetamide (<0.1%) 500 µ L recomWell HEV IgM also contains: 12 x 8 wells microplate (section marked in green), coated with recombinant HEV antigens in a vacuum-pressure-sealed bag 1 Positive control (black cap) Contains MIT (0.1%) and Oxypyrion (0.1%) 450 µ L Cutoff control (colourless cap) Contains MIT (0.1%) and Oxypyrion (0.1%) 450 µ L Negative control (white cap) Contains MIT (0.1%) and Oxypyrion (0.1%) 450 µ L Anti-human IgM conjugate (101x concentrated, green cap) Contains NaN? (<0.1%), MIT (<0.01%) and chlorazetamide (<0.1%) 500 µ L
Storage Store at 2-8°C.

target relevance
Organism Campylobacter jejuni
Structure and strains Campylobacter jejuni is a species of pathogenic bacteria, one of the most common causes of food poisoning in Europe and in the US. The vast majority of cases occur as isolated events, not as part of recognized outbreaks. Active surveillance through the Foodborne Diseases Active Surveillance Network (FoodNet) indicates that about 20 cases are diagnosed each year for each 100,000 people in the US, while many more cases are undiagnosed or unreported; the CDC estimates a total of 1.5 million infections every year. The European Food Safety Authority reported 246,571 cases in 2018, and estimated approximately nine million cases of human campylobacteriosis per year in the European Union.
Disease Campylobacter is a bacteria consisting of more than 20 species occuring worldwide. In particular, Campylobacter jejuni is associated with human diseases in industrialized countries. The main reservoirs of the are warmblooded domestic and wild animals. Human Campylobacteriosis is primarily a foodborne disease. Insufficiently heated, contaminated poultry and poultry products (eggs excluded) are the main sources of infection. A clinical manifestation of Campylobacter jejuni infections is acute enteritis which is accompanied by fever (38 - 40°C), headache, myalgia, anthralgia and weariness. Normally infections are self limiting, but 5 to 10% of untreated patients develop relapses. Rare complications of infection are Guillain-Barre-Syndrome, an inflammatory disease of the peripheral nervous system, and reactive arthritis. Diagnosis Campylobacter jejuni infections are usually detected by pathogen isolations from stool and blood samples. I
Detection and diagnosis Campylobacter jejuni infections are usually detected by pathogen isolations from stool and blood samples. In recent years, serological diagnosis of Campylobacter jejuni infections has developed into an important routine diagnostic procedure. To analyze the etiology of severe complications, such as reactive arthritis and GBS, reliable serological ELISA tests are required, since these diseases usually develop up to three weeks after Campylobacter jejuni infection. In such cases, stool culture testing is not suitable since isolation attempts are usually unsuccessful at this stage.