Western blotting is a powerful, tried-and trusted technique for studying proteins for more information about experimental design see our guide, but even the most seasoned researchers encounter challenges along the way. In this guide, we’ll explore common troubleshooting issues associated with Western blotting and share… Read More »Blotting Out Uncertainties: Troubleshooting Common Western Blotting Issues
Introduction: Western blotting is a powerful, tried-and trusted technique for studying proteins for more information about experimental design see our guide. In this blog post, we will delve into the science behind nitrocellulose’s role in protein research, explore how it works in this context, uncover its… Read More »Nitrocellulose in Protein Research: An Explosive Blend of Science and History
In the world of molecular biology and biomedical research, antibody-horseradish peroxidase (HRP) conjugates have emerged as indispensable tools for various applications, including immunoassays, immunohistochemistry, and Western blotting. To appreciate their significance fully, one must delve into the chemistry of the peroxidase reaction that powers these conjugates. In this blog post, we’ll explore HRP chemistry, shedding light on the catalytic process that makes antibody-HRP conjugates invaluable in the laboratory.
Non-specific protein blocking is a crucial step in various molecular biology and immunoassay techniques, including Western blotting and immunohistochemistry. It involves the pre-treatment of a biological sample or a membrane to prevent non-specific binding of antibodies or other detection molecules to unwanted proteins or surfaces.… Read More »Surface and membrane blocking
In the realm of molecular biology and protein research, the transfer of proteins from an acrylamide gel onto a nitrocellulose membrane is a pivotal step that opens up a world of possibilities for further analysis. This technique, known as Western blotting or immunoblotting, allows scientists… Read More »The Science of Transferring Proteins: From Acrylamide Gel onto Nitrocellulose