HPLC of Varicella-zoster virus (strain Oka vaccine) Envelope glycoprote 2851

SDS-PAGE gel of Varicella-zoster virus (strain Oka vaccine) Envelope gl 2851

Varicella-zoster virus (strain Oka vaccine) Envelope glycoprotein E Protein 2851

$240.00 – $800.00

Summary

Expression: HEK293
Pure: Yes (HPLC)
Amino Acid Range: Ser31-Tyr538

SKU: 2851parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q9J3M8
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
background	VZV glycoprotein E (gE) is most abundantly expressed on the surface of infected cells, and is an essential component for virus replication and cell-to-cell transmission. It is also the main target of virus-specific antibodies and T cell responses that is often selected as vaccine candidate antigen.
molecular weight	The protein has a predicted MW of 58.39 kDa. Due to glycosylation, the protein migrates to 65-85 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1 EU per μg by the LAL method.

Varicella-zoster virus (strain Oka vaccine) Envelope glycoprotein E Protein 2851

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
VZV glycoprotein E (gE) is most abundantly expressed on the surface of infected cells, and is an essential component for virus replication and cell-to-cell transmission. It is also the main target of virus-specific antibodies and T cell responses that is often selected as vaccine candidate antigen.
Protein names Envelope glycoprotein E (gE)
Gene names gE,gE ORF68
Protein family Alphaherpesvirinae glycoprotein E family
Mass 341980Da
Function Envelope glycoprotein that binds to the potential host cell entry receptor IDE.; In epithelial cells, the heterodimer gE/gI is required for the cell-to-cell spread of the virus, by sorting nascent virions to cell junctions. Once the virus reaches the cell junctions, virus particles can spread to adjacent cells extremely rapidly through interactions with cellular receptors that accumulate at these junctions. Implicated in basolateral spread in polarized cells. In neuronal cells, gE/gI is essential for the anterograde spread of the infection throughout the host nervous system. Together with US9, the heterodimer gE/gI is involved in the sorting and transport of viral structural components toward axon tips (By similarity).; The heterodimer gE/gI serves as a receptor for the Fc part of host IgG. Dissociation of gE/gI from IgG occurs at acidic pH. May thus be involved in anti-VZV antibodies bipolar bridging, followed by intracellular endocytosis and degradation, thereby interfering with host IgG-mediated immune responses (By similarity).
Subellular location Virion membrane ; Single-pass type I membrane protein. Host cell membrane ; Single-pass type I membrane protein. Host cell junction. Host Golgi apparatus membrane ; Single-pass membrane protein. Host endosome membrane ; Single-pass membrane protein. Note=During virion morphogenesis, this protein probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is probably transported to the cell surface from where it is endocytosed and directed to the trans-Golgi network (TGN), maybe through an interaction with PACS-1 sorting protein. The heterodimer gE/gI then redistributes to cell junctions to promote cell-cell spread later in the infection (By similarity).
Structure Interacts (via N-terminus) with host receptor IDE (via N-terminus). Interacts with gI; this interaction enhances the Fc receptor function of gE (By similarity). The heterodimer gE/gI interacts with the Fc part of host IgG (By similarity).
Post-translational modification Phosphorylated on serines within the acidic cluster. Phosphorylation determines whether endocytosed viral gE traffics to the trans-Golgi network or recycles to the cell membrane.
Target Relevance information above includes information from UniProt accession: Q9J3M8
The UniProt Consortium

HPLC of Varicella-zoster virus (strain Oka vaccine) Envelope glycoprote

The purity of Varicella-zoster virus (strain Oka vaccine) Envelope glycoprotein E is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Varicella-zoster virus (strain Oka vaccine) Envelope gl

Varicella-zoster virus (strain Oka vaccine) Envelope glycoprotein E on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.