ELISA with SARS-COV-2 Spike S1 (N501Y) Protein 4376

SARS-COV-2 Spike S1 (N501Y) Protein 4376

$300.00 – $1,000.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Val16-Arg685

SKU: 4376parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	YP_009724390
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	A new variant of SARS-CoV-2 is spreading in the UK and is rapidly becoming a global threat.It is characterised by multiple mutations in the spike protein. Among them, N501Y is of major concern because it involves one of the six key amino acid residues determining a tight interaction of the SARS-CoV-2 receptor-binding domain (RBD) with its cellular receptor angiotensin-converting enzyme 2 (ACE2).
molecular weight	The protein has a predicted MW of 76 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

SARS-COV-2 Spike S1 (N501Y) Protein 4376

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

No information

target relevance

Data

ELISA with SARS-COV-2 Spike S1 (N501Y) Protein

Immobilized SARS-COV-2 Spike S1 (N501Y) , His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 66.2ng/ml determined by ELISA.

HPLC of SARS-COV-2 Spike S1 (N501Y) Protein

The purity of SARS-COV-2 Spike S1 (N501Y) is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of SARS-COV-2 Spike S1 (N501Y) Protein

SARS-COV-2 Spike S1 (N501Y) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.