SPR with SARS-COV-2 Spike RBD (N501Y) Protein 4377

ELISA with SARS-COV-2 Spike RBD (N501Y) Protein 4377

SARS-COV-2 Spike RBD (N501Y) Protein 4377

$345.00 – $1,150.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Arg319-Phe541(N501Y)

SKU: 4377parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	QHD43416
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation. Deep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. In particular, the N501Y mutation is located at the receptor binding domain (RBD) of the spike protein.
molecular weight	The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

SARS-COV-2 Spike RBD (N501Y) Protein 4377

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

No information

target relevance

Data

SPR with SARS-COV-2 Spike RBD (N501Y) Protein

Human ACE2 captured on Protein A chip, can bind SARS-COV-2 Spike RBD (N501Y) , His Tag with an affinity constant of 1.74nM as determined in a SPR assay (Biacore T200).

ELISA with SARS-COV-2 Spike RBD (N501Y) Protein

Immobilized SARS-COV-2 Spike RBD (N501Y) , His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test).

HPLC of SARS-COV-2 Spike RBD (N501Y) Protein

The purity of SARS-COV-2 Spike RBD (N501Y) is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of SARS-COV-2 Spike RBD (N501Y) Protein

SARS-COV-2 Spike RBD (N501Y) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.