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RSV (B) Pre-fusion glycoprotein F0 Protein 2255

$675.00$2,250.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range:
SKU: 2255parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

0

express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Respiratory syncytial virus (RSV) is a highly contagious childhood pathogen of the respiratory tract and is divided into two antigenic subtypes, A and B, based on the reactivity of the F and G surface proteins to monoclonal antibodies. Surface protein F (fusion protein) is responsible for fusion of viral and host cell membranes, as well as syncytium formation between viral particles. Its sequence is highly conserved between strains. F protein exists in multiple conformational forms. In the prefusion state (PreF), the protein exists in a trimeric form and contains the major antigenic site Ø which serves as a primary target of neutralizing antibodies in the body.

molecular weight

The protein has a predicted MW of 56.50 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

RSV (B) Pre-fusion glycoprotein F0 Protein 2255

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
No information
target relevance

ELISA with RSV (B) Pre-fusion glycoprotein F0 Protein
Immobilized Anti-Fusion glycoprotein F0 (D25) Antibody, hFc Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for RSV (B) Pre-fusion glycoprotein F0, His Tag with the EC50 of 6.9ng/ml determined by ELISA (QC Test).
ELISA with RSV (B) Pre-fusion glycoprotein F0 Protein
Immobilized RSV (B) Pre-fusion glycoprotein F0, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (101F) Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.
HPLC of RSV (B) Pre-fusion glycoprotein F0 Protein
The purity of RSV (B) Pre-fusion glycoprotein F0 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of RSV (B) Pre-fusion glycoprotein F0 Protein
RSV (B) Pre-fusion glycoprotein F0 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

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We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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