Rhesus macaque Latent TGF beta 1/TGFB1 Protein 4714

$405.00 – $1,350.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Leu30-Ser390(C33S)

available size

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SKU: 4714parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	F7HCV5
express system	HEK293
product tag	N-His
purity	> 95% as determined by Tris-Bis PAGE
background	Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1. Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex.
molecular weight	The protein has a predicted MW of 31.4/12.8 kDa. Due to glycosylation, the protein migrates to 40-50/14-15 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Rhesus macaque Latent TGF beta 1/TGFB1 Protein 4714

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Latent TGF beta 1Ã‚Â cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF- beta 1 remain non-covalently associated after secretion, forming the small latent TGF- beta 1 complex.
Protein names Transforming growth factor beta
Gene names TGFB1,TGFB1
Protein family TGF-beta family
Mass 44297Da
Function FUNCTION: Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1. Interaction with LRRC33/NRROS regulates activation of TGF-beta-1 in macrophages and microglia. Interaction with LRRC32/GARP controls activation of TGF-beta-1 on the surface of activated regulatory T-cells (Tregs). Interaction with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1. {ECO:0000256\|ARBA:ARBA00003201}.; FUNCTION: Transforming growth factor beta-1 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively. {ECO:0000256\|ARBA:ARBA00002007}.
Subellular location SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix {ECO:0000256\|ARBA:ARBA00004498}.
Structure SUBUNIT: Homodimer; disulfide-linked. Interacts with TGF-beta receptors (TGFBR1 and TGFBR2), leading to signal transduction. {ECO:0000256\|ARBA:ARBA00046998}.
Target Relevance information above includes information from UniProt accession: F7HCV5
The UniProt Consortium

Data

ELISA with Rhesus macaque Latent TGF beta 1/TGFB1 Protein

Immobilized Rhesus macaque Latent TGF beta 1, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 0.74µg/ml determined by ELISA.

SDS-PAGE gel of Rhesus macaque Latent TGF beta 1/TGFB1 Protein

Rhesus macaque Latent TGF beta 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.