ELISA with Rhesus macaque IL-18 R1/CD218a Protein 2305

Rhesus macaque IL-18 R1/CD218a Protein 2305

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Cys22-Gly329

SKU: 2305parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	F7H8F9
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Interleukin-18 (IL-18) is a member of the interleukin-1 family of cytokines produced constitutively by different cell types and by adipose tissue. Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
molecular weight	The protein has a predicted MW of 61.96 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Rhesus macaque IL-18 R1/CD218a Protein 2305

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Interleukin-18 (IL-18) is a member of the interleukin-1 family of cytokines produced constitutively by different cell types and by adipose tissue. Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naÃ¯ve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
Protein names Interleukin 18 receptor 1
Gene names IL18R1,IL18R1
Protein family Interleukin-1 receptor family
Mass 9544Da
Target Relevance information above includes information from UniProt accession: F7H8F9
The UniProt Consortium

Data

ELISA with Rhesus macaque IL-18 R1/CD218a Protein

Immobilized Rhesus macaque IL-18 R1, hFc Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 0.42µg/ml determined by ELISA.

HPLC of Rhesus macaque IL-18 R1/CD218a Protein

The purity of Rhesus macaque IL-18 R1 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Rhesus macaque IL-18 R1/CD218a Protein

Rhesus macaque IL-18 R1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.