| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| accession | O02948(HLA-G)&Q6V7J5(B2M)&RIIPRHLQL |
| express system | HEK293 |
| product tag | C-His-Avi |
| purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| background | HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition. |
| molecular weight | The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 58-63 kDa based on Tris-Bis PAGE result. |
| available size | 100 µg, 500 µg |
| endotoxin | Less than 1EU per μg by the LAL method. |
Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein 3232
$310.00 – $1,125.00
Summary
- Expression: HEK293
- Functional: Yes (ELISA)
- Amino Acid Range: Gly25-Thr305(HLA-G), Ile21-Met119(B2M) and RIIPRHLQL peptide
Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein 3232
| protein |
|---|
| Size and concentration 100, 500µg and lyophilized |
| Form Lyophilized |
| Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| Storage buffer Shipped at ambient temperature. |
| Purity > 95% as determined by Tris-Bis PAGE |
| target relevance |
|---|
| HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition. |
| Protein names MHC class I antigen |
| Gene names Mamu-AG,Mamu-AG |
| Protein family MHC class I family |
| Mass 9544Da |
| Function Involved in the presentation of foreign antigens to the immune system. |
| Subellular location Membrane ; Single-pass type I membrane protein . |
| Target Relevance information above includes information from UniProt accession: O02948 |
| The UniProt Consortium |
 |
| Rhesus macaque LILRB1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag with an affinity constant of 27.18 nM as determined in SPR assay (Biacore T200). |
 |
| Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 1.4µg/ml determined by ELISA (QC Test). |
 |
| The purity of Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer is greater than 95% as determined by SEC-HPLC. |
 |
| Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
Frequently Asked Questions
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.
1. What are the key features of the Rhesus Macaque HLA-G/B2M Peptide (RIIPRHLQL) Monomer Protein?
- This monomer protein is specifically designed for studying immune responses in Rhesus Macaque models.
- It consists of the HLA-G complexed with B2M and the peptide RIIPRHLQL, mimicking a natural presentation of antigens.
- The protein is highly pure and biologically active, making it suitable for various immunological assays.
2. How should I store and handle the Rhesus Macaque HLA-G/B2M Peptide (RIIPRHLQL) Monomer Protein?
- The protein should be stored at -80°C upon arrival to maintain its stability and activity.
- It is recommended to aliquot the protein in smaller volumes to avoid repeated freeze-thaw cycles that may affect its integrity.
- When handling the protein, it is important to use proper protective equipment and sterile techniques to prevent contamination.
3. Is the Rhesus Macaque HLA-G/B2M Peptide (RIIPRHLQL) Monomer Protein compatible with flow cytometry and other assay techniques?
- Yes, this monomer protein is compatible with flow cytometry and other immunological assays commonly used in research settings.
- It can be used to study T cell responses, antigen presentation, and immune tolerance in Rhesus Macaque models.
- Researchers can utilize this protein to characterize immune responses and investigate interactions between antigens and T cell receptors.
4. Can the Rhesus Macaque HLA-G/B2M Peptide (RIIPRHLQL) Monomer Protein be used for in vitro or in vivo studies?
- This monomer protein is primarily designed for in vitro studies to assess immune responses and T cell activation.
- It can be used to investigate antigen-specific cellular responses in cultured cells or ex vivo samples.
- While in vivo applications may be possible, researchers should assess compatibility and biological activity in their specific experimental models.
5. What are the potential applications of the Rhesus Macaque HLA-G/B2M Peptide (RIIPRHLQL) Monomer Protein in research?
- This protein can be used in research related to transplantation, infectious diseases, cancer immunotherapy, and autoimmune disorders.
- It allows researchers to characterize immune responses in Rhesus Macaque models and address questions related to antigen presentation and T cell recognition.
- The monomer protein serves as a valuable tool for understanding immune tolerance, immune evasion mechanisms, and T cell receptor specificity in non-human primate research.
- This monomer protein is specifically designed for studying immune responses in Rhesus Macaque models.
- It consists of the HLA-G complexed with B2M and the peptide RIIPRHLQL, mimicking a natural presentation of antigens.
- The protein is highly pure and biologically active, making it suitable for various immunological assays.
2. How should I store and handle the Rhesus Macaque HLA-G/B2M Peptide (RIIPRHLQL) Monomer Protein?
- The protein should be stored at -80°C upon arrival to maintain its stability and activity.
- It is recommended to aliquot the protein in smaller volumes to avoid repeated freeze-thaw cycles that may affect its integrity.
- When handling the protein, it is important to use proper protective equipment and sterile techniques to prevent contamination.
3. Is the Rhesus Macaque HLA-G/B2M Peptide (RIIPRHLQL) Monomer Protein compatible with flow cytometry and other assay techniques?
- Yes, this monomer protein is compatible with flow cytometry and other immunological assays commonly used in research settings.
- It can be used to study T cell responses, antigen presentation, and immune tolerance in Rhesus Macaque models.
- Researchers can utilize this protein to characterize immune responses and investigate interactions between antigens and T cell receptors.
4. Can the Rhesus Macaque HLA-G/B2M Peptide (RIIPRHLQL) Monomer Protein be used for in vitro or in vivo studies?
- This monomer protein is primarily designed for in vitro studies to assess immune responses and T cell activation.
- It can be used to investigate antigen-specific cellular responses in cultured cells or ex vivo samples.
- While in vivo applications may be possible, researchers should assess compatibility and biological activity in their specific experimental models.
5. What are the potential applications of the Rhesus Macaque HLA-G/B2M Peptide (RIIPRHLQL) Monomer Protein in research?
- This protein can be used in research related to transplantation, infectious diseases, cancer immunotherapy, and autoimmune disorders.
- It allows researchers to characterize immune responses in Rhesus Macaque models and address questions related to antigen presentation and T cell recognition.
- The monomer protein serves as a valuable tool for understanding immune tolerance, immune evasion mechanisms, and T cell receptor specificity in non-human primate research.
Publications
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| We haven't added any publications to our database yet. | |||
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