ELISA with Rhesus macaque CD4/LEU3 Protein 5170

Rhesus macaque CD4/LEU3 Protein 5170

$225.00 – $750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Lys26-Trp390

SKU: 5170parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	G7N5T8
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.
molecular weight	The protein has a predicted MW of 41.5 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Rhesus macaque CD4/LEU3 Protein 5170

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.
Protein names T-cell surface glycoprotein CD4 (T-cell surface antigen T4/Leu-3)
Gene names CD4,CD4 EGK_03289
Mass 9544Da
Subellular location Cell membrane ; Single-pass type I membrane protein. Membrane ; Single-pass type I membrane protein .
Structure Forms disulfide-linked homodimers at the cell surface. Interacts with LCK. Interacts with PTK2/FAK1. Binds to P4HB/PDI. Interacts with IL16; this interaction induces a CD4-dependent signaling in lymphocytes. Interacts (via Ig-like V-type domain) with MHCII alpha chain (via alpha-2 domain) and beta chain (via beta-2 domain); this interaction increases the affinity of TCR for peptide-MHCII. CD4 oligomerization via Ig-like C2-type 2 and 3 domains appears to be required for stable binding to MHCII and adhesion between T cells and APCs.
Target Relevance information above includes information from UniProt accession: G7N5T8
The UniProt Consortium

Data

ELISA with Rhesus macaque CD4/LEU3 Protein

Immobilized Rhesus macaque CD4, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA.

The purity of Rhesus macaque CD4 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Rhesus macaque CD4/LEU3 Protein

Rhesus macaque CD4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.