ELISA with Rat GARP&Latent TGF Beta 1 Complex Protein 3224

Rat GARP&Latent TGF Beta 1 Complex Protein 3224

$450.00 – $1,500.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ile18-Asn628(GARP) & Leu30-Arg278(Latent TGF Beta 1)

SKU: 3224parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	D3ZVD5(GARP)&P17246(Latent TGF Beta)
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
molecular weight	The protein has a predicted MW of 69.80 kDa (GARP)&41.28 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&42-48 kDa&13 kDa (Latent TGF beta 1) based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Rat GARP&Latent TGF Beta 1 Complex Protein 3224

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
Protein names Leucine rich repeat containing 32
Gene names Lrrc32,Lrrc32
Mass 72684Da
Target Relevance information above includes information from UniProt accession: D3ZVD5
The UniProt Consortium

Data

ELISA with Rat GARP&Latent TGF Beta 1 Complex Protein

Immobilized Rat GARP&Latent TGF Beta 1 Complex, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 1.44µg/ml determined by ELISA.

HPLC of Rat GARP&Latent TGF Beta 1 Complex Protein

The purity of Rat GARP&Latent TGF Beta 1 Complex is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Rat GARP&Latent TGF Beta 1 Complex Protein

Rat GARP&Latent TGF Beta 1 Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

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Frequently Asked Questions

1. Q: What are the key features of the Rat GARP/Latent TGF-beta 1 Complex Protein (3224)?
A: The Rat GARP/Latent TGF-beta 1 Complex Protein (3224) is a recombinant protein that allows for the study of TGF-beta 1 activation and downstream signaling pathways. It is specifically designed for research purposes and is produced in a mammalian expression system for high quality and authenticity.

2. Q: How should I store the Rat GARP/Latent TGF-beta 1 Complex Protein (3224)?
A: To maintain the stability and integrity of the protein, it is recommended to store the Rat GARP/Latent TGF-beta 1 Complex Protein (3224) at -80°C upon arrival. Avoid repeated freeze-thaw cycles to prevent degradation.

3. Q: Is this protein compatible with common laboratory techniques and assays?
A: Yes, the Rat GARP/Latent TGF-beta 1 Complex Protein (3224) is compatible with various laboratory techniques such as Western blotting, ELISA, immunoprecipitation, and functional studies involving TGF-beta 1 signaling pathways. It can be used in a wide range of applications for studying TGF-beta 1 biology.

4. Q: What are the potential applications of the Rat GARP/Latent TGF-beta 1 Complex Protein (3224)?
A: The Rat GARP/Latent TGF-beta 1 Complex Protein (3224) can be used in research related to TGF-beta 1 biology, including investigating TGF-beta 1 activation mechanisms, studying its effects on cell signaling pathways, and exploring its role in various cellular processes such as differentiation, proliferation, and immune response modulation.

5. Q: How can I use the Rat GARP/Latent TGF-beta 1 Complex Protein (3224) in my experiments?
A: The Rat GARP/Latent TGF-beta 1 Complex Protein (3224) can be used as a positive control in experiments to validate TGF-beta 1 activation assays or as a research tool to investigate the functions and mechanisms of TGF-beta 1 signaling. Detailed protocols for incorporating the protein into various experimental setups can be found in the provided datasheet or user manual.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

We havent added any publications to our database yet.